Anti-HARS antibody (ab77034)


  • Product nameAnti-HARS antibody
    See all HARS primary antibodies
  • Description
    Mouse monoclonal to HARS
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster, Cow, Cynomolgus monkey, Orangutan
  • Immunogen

    Recombinant fragment: MAERAALEEL VKLQGERVRG LKQQKASAEL IEEEVAKLLK LKAQLGPDES KQKFVLKTPK GTRDYSPRQM AVREKVFDVI IRCFKRHGAE VIDTPV, corresponding to amino acids 1-96 of human HARS (NP_002100) with a 26 kDa tag

  • Positive control
    • HeLa cytoplasmic lysate; partial recombinant human HARS (the immunogen).



Our Abpromise guarantee covers the use of ab77034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
ELISA Use at an assay dependent dilution. Use HRP conjugated goat anti-rabbit IgG (H+L) as a secondary antibody. Detection limit for recombinant HARS is approximately 0.3 ng/ml as a capture antibody.


  • Tissue specificityBrain, heart, liver and kidney.
  • Involvement in diseaseDefects in HARS are a cause of Usher syndrome type 3B (USH3B) [MIM:614504]. USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life.
  • Sequence similaritiesBelongs to the class-II aminoacyl-tRNA synthetase family.
    Contains 1 WHEP-TRS domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic antibody
    • EC antibody
    • FLJ20491 antibody
    • HARS antibody
    • HisRS antibody
    • Histidine tRNA ligase, cytoplasmic antibody
    • histidine translase antibody
    • Histidine tRNA ligase antibody
    • Histidine--tRNA ligase antibody
    • Histidyl tRNA synthetase antibody
    • Histidyl-tRNA synthetase antibody
    • HRS antibody
    • Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds antibody
    • SYHC_HUMAN antibody
    • USH3B antibody
    see all

Anti-HARS antibody images

  • Anti-HARS antibody (ab77034) at 1/500 dilution + HeLa cell lysate at 25 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 56 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Anti-HARS antibody (ab77034) at 1/500 dilution + partial recombinant human HARS protein at 0.2 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 56 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Sandwich ELISA using ab77034 at 1µg/mL, 100µL/well and recombinant HARS protein. HRP conjugated goat anti-rabbit IgG (H+L) used as secondary antibody.

References for Anti-HARS antibody (ab77034)

ab77034 has not yet been referenced specifically in any publications.

Product Wall

There is no clone number for ab77034, anti-HARS. If there is a clone number for any of our products, then this is clearly stated on the datasheet for the products. If there is anything else I can help you with, please let me know.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Recombinant protein (purified protein)
Specification purified protein
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Type Sandwich (Detection)

Abcam user community

Verified customer

Submitted Feb 25 2010