Overview

  • Product name
    Anti-HARS2 antibody - C-terminal
  • Description
    Rabbit polyclonal to HARS2 - C-terminal
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human HARS2 conjugated to KLH

  • Positive control
    • Hela cell line lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab71352 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
IHC-P 1/10 - 1/50.

Target

  • Tissue specificity
    A high level expression is seen in the heart, kidney and skeletal muscle while a lower level expression is seen in the brain and liver.
  • Sequence similarities
    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4631412B19Rik antibody
    • AI593507 antibody
    • HARS 2 antibody
    • HARS related antibody
    • Hars2 antibody
    • Harsl antibody
    • HARSR antibody
    • HisRS antibody
    • Histidine translase antibody
    • Histidine tRNA ligase antibody
    • Histidine tRNA ligase homolog antibody
    • Histidine tRNA ligase like antibody
    • Histidine--tRNA ligase antibody
    • Histidine--tRNA ligase-like antibody
    • Histidyl tRNA synthetase 2 mitochondrial antibody
    • Histidyl tRNA synthetase like antibody
    • HO3 antibody
    • mitochondrial antibody
    • PRLTS2 antibody
    • Probable histidyl tRNA synthetase mitochondrial antibody
    • Probable histidyl-tRNA synthetase antibody
    • SYHM_HUMAN antibody
    see all

Images

  • Anti-HARS2 antibody - C-terminal (ab71352) at 1/60 dilution + Hela cell line lysate at 35 µg

    Predicted band size : 57 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab71352 at 1/50 dilution staining HARS2 - C - terminal in human testis tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References

ab71352 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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