Overview

  • Product nameAnti-Haspin antibody
    See all Haspin primary antibodies
  • Description
    Rabbit polyclonal to Haspin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Haspin.

    (Peptide available as ab23532.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa Expressing myc-Haspin (interphase); HeLa Expressing myc-Haspin (mitosis) Whole Cell Lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).

Target

  • FunctionRequired for normal alignment of chromosomes at metaphase. Phosphorylates histone H3 'Thr-3' during mitosis.
  • Tissue specificityStrongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on both serine and threonine residues (By similarity). Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • germ cell associated 2 antibody
    • Germ cell-specific gene 2 protein antibody
    • GSG2 antibody
    • H-haspin antibody
    • Haploid germ cell-specific nuclear protein kinase antibody
    • HASP_HUMAN antibody
    • Serine/threonine-protein kinase haspin antibody
    see all

Anti-Haspin antibody images

  • All lanes : Anti-Haspin antibody (ab21686) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 2 : HeLa Expressing myc-Haspin (interphase) Whole Cell Lysate - Positive Control at 5 µl
    Lane 3 : HeLa Interphase Whole Cell Lysate - Negative Control at 10 µg
    Lane 4 : HeLa Expressing myc-Haspin (mitosis) Whole Cell Lysate - Positive Control at 5 µl
    Lane 5 : HeLa Mitosis Whole Cell Lysate - Negative Control at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 88 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References for Anti-Haspin antibody (ab21686)

This product has been referenced in:
  • Varier RA  et al. A phospho/methyl switch at histone H3 regulates TFIID association with mitotic chromosomes. EMBO J 29:3967-78 (2010). Read more (PubMed: 20953165) »
  • Markaki Y  et al. Phosphorylation of histone H3 at Thr3 is part of a combinatorial pattern that marks and configures mitotic chromatin. J Cell Sci 122:2809-19 (2009). WB ; Human, Mouse . Read more (PubMed: 19622635) »

See all 3 Publications for this product

Product Wall

Thank you for contacting Abcam regarding ab21686. I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information provided and would like to make some suggestions to improve the results wit...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 17 µg
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Jonathan Higgins

Verified customer

Submitted Feb 12 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"