Overview

  • Product name
    Anti-HAUS4 antibody
  • Description
    Rabbit polyclonal to HAUS4
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 296-326 of Human HAUS4.

  • Positive control
    • MCF7 cell line lysates. Skin carcinoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    This gene encodes a subunit of the centrosome complex termed the human augmin complex. The encoded protein localizes to the spindle microtubules and may play a role in mitotic spindle assembly and maintenance of centrosome integrity during cell division. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1.
  • Cellular localization
    Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton › spindle.
  • Database links
  • Alternative names
    • C14orf94 antibody
    • Chromosome 14 open reading frame 94 antibody
    • FLJ20424 antibody
    • FLJ40661 antibody
    • HAUS augmin like complex subunit 4 antibody
    • Homologous to Augmin subunits antibody
    • OTTHUMP00000164554 antibody
    see all

Anti-HAUS4 antibody images

  • Anti-HAUS4 antibody (ab103709) at 1/100 dilution + MCF7 cell line lysates at 35 µg

    Predicted band size : 42 kDa
  • ab103709 at 1/50 dilution staining HAUS4 in formalin-fixed and paraffin-embedded skin carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised DAB.

References for Anti-HAUS4 antibody (ab103709)

ab103709 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (Spindles in X. laevis cytoplasmic egg extract)
Specification
Spindles in X. laevis cytoplasmic egg extract
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - other (Xenopus laevis cytoplasmic egg extract)
Loading amount
50 µg
Specification
Xenopus laevis cytoplasmic egg extract
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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