Overview

  • Product nameAnti-HAUS5 antibody
  • Description
    Rabbit polyclonal to HAUS5
  • Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human HAUS5 aa 139-167 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • Human colon carcinoma.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceHAUS5 is 1 of 8 subunits of the 390-kD Human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly.
  • Cellular localizationCytoplasm, cytoskeleton, centrosome and spindle.
  • Database links
  • Alternative names
    • Dgt5 antibody
    • HAUS augmin like complex subunit 5 antibody
    • HAUS augmin like complex, subunit 5 antibody
    • KIAA0841 antibody

Anti-HAUS5 antibody images

  • HAUS5 Antibody (N-term) (ab103721) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • ab103721 at 1/50 dilution staining HAUS5 by immunohistochemistry in formalin fixed and paraffin embedded Human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

References for Anti-HAUS5 antibody (ab103721)

ab103721 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (Spindles in X. laevis cytoplasmic egg extract)
Specification Spindles in X. laevis cytoplasmic egg extract
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Oct 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - other (Xenopus laevis cytoplasmic egg extract)
Loading amount 50 µg
Specification Xenopus laevis cytoplasmic egg extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"