Overview

  • Product name
    Anti-HAUSP / USP7 antibody
    See all HAUSP / USP7 primary antibodies
  • Description
    Rabbit polyclonal to HAUSP / USP7
  • Specificity
    By Western blot, this antibody detects a single clean band of about 130kD representing HAUSP in various cell lysates (see picture). The band is strongly depleted using peptide blocking with the immunising peptide.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    TDDPPRITQNPVC

    , corresponding to amino acids 30-41 of Human HAUSP.

Properties

Applications

Our Abpromise guarantee covers the use of ab4080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/15000 - 1/60000.
WB 1/500 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration.
IHC-P 1/500. PubMed: 16450335

Target

  • Function
    Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (PubMed:25283148). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection. Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (PubMed:21745816). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed:23973222).
  • Tissue specificity
    Widely expressed. Overexpressed in prostate cancer.
  • Sequence similarities
    Belongs to the peptidase C19 family.
    Contains 1 MATH domain.
    Contains 1 USP domain.
  • Domain
    The C-terminus plays a role in its oligomerization.
  • Post-translational
    modifications
    Isoform 1: Phosphorylated. Isoform 1 is phosphorylated at positions Ser-18 and Ser-963. Isoform 2: Not phosphorylated.
    Isoform 1: Polyneddylated. Isoform 2: Not Polyneddylated.
    Isoform 1 and isoform 2: Not sumoylated.
    Isoform 1 and isoform 2: Polyubiquitinated by herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110; leading to its subsequent proteasomal degradation. Isoform 1: Ubiquitinated at Lys-869.
  • Cellular localization
    Nucleus. Cytoplasm. Nucleus, PML body. Present in a minority of ND10 nuclear bodies. Association with ICP0/VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus. Colocalized with DAXX in speckled structures. Colocalized with PML and PTEN in promyelocytic leukemia protein (PML) nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 7 antibody
    • HAUSP antibody
    • Herpes virus associated ubiquitin specific protease antibody
    • Herpesvirus-associated ubiquitin-specific protease antibody
    • TEF 1 antibody
    • tef-1 antibody
    • TEF1 antibody
    • Ubiquitin carboxyl terminal hydrolase 7 antibody
    • Ubiquitin carboxyl-terminal hydrolase 7 antibody
    • Ubiquitin specific peptidase 7 (herpes virus associated) antibody
    • Ubiquitin specific peptidase 7 antibody
    • Ubiquitin specific peptidase 7 herpes virus associated antibody
    • Ubiquitin specific processing protease 7 antibody
    • Ubiquitin specific protease 7 (herpes virus associated) antibody
    • Ubiquitin specific protease 7 antibody
    • Ubiquitin specific protease 7 herpes virus associated antibody
    • Ubiquitin thioesterase 7 antibody
    • Ubiquitin thiolesterase 7 antibody
    • Ubiquitin-specific-processing protease 7 antibody
    • UBP 7 antibody
    • UBP-7 antibody
    • UBP7 antibody
    • UBP7_HUMAN antibody
    • USP 7 antibody
    • usp-7 antibody
    • Usp7 antibody
    • VMW110-ASSOCIATED PROTEIN, 135-KD antibody
    see all

Images



  • Predicted band size : 128 kDa

    Western blot using ab4080 at 1/500. Secondary antibody anti-rabbit HRP ab6721 at 1/5000. Exposure time 30s.

    Lane 1: Hela nuclear
    Lane 2: Hela
    Lane 3: A431
    Lane 4: MCF7
    Lane 5: 3T3
    Lane 6: Hela nuclear + ab11707 HAUSP peptide (aa30-41)
    Lane 7: Hela + ab11707 HAUSP peptide (aa30-41)
    Lane 8: A431 + ab11707 HAUSP peptide (aa30-41)
    Lane 9: MCF7 + ab11707 HAUSP peptide (aa30-41)
    Lane 10: 3T3 + ab11707 HAUSP peptide (aa30-41)

    Western blot using ab4080 at 1/500. Secondary ab6721 at 1/5000. Exposure time 30s.

    Lane 1: Hela nuclear
    Lane 2: Hela
    Lane 3: A431
    Lane 4: MCF7
    Lane 5: 3T3
    Lane 6: Hela nuclear + ab11707 HAUSP peptide (aa30-41)
    Lane 7: Hela + ab11707 HAUSP peptide (aa30-41)
    Lane 8: A431 + ab11707 HAUSP peptide (aa30-41)
    Lane 9: MCF7 + ab11707 HAUSP peptide (aa30-41)
    Lane 10: 3T3 + ab11707 HAUSP peptide (aa30-41)

  • ICC/IF image of ab4080 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4080, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Ma M & Yu N Ubiquitin-specific protease 7 expression is a prognostic factor in epithelial ovarian cancer and correlates with lymph node metastasis. Onco Targets Ther 9:1559-69 (2016). WB, IHC . Read more (PubMed: 27051296) »
  • Chen ST  et al. The Deubiquitinating Enzyme USP7 Regulates Androgen Receptor Activity by Modulating Its Binding to Chromatin. J Biol Chem 290:21713-23 (2015). Read more (PubMed: 26175158) »

See all 9 Publications for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (HCT116)
Specification
HCT116
Total protein in input
50 µg
Username

Abcam user community

Verified customer

Submitted Feb 18 2014

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (Gradient Gel)
Sample
Human Cell lysate - whole cell (Different Breast Cancer Lines - MDAMB231 453 Cal12)
Specification
Different Breast Cancer Lines - MDAMB231 453 Cal12
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin, muscle)
Specification
skin, muscle
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
casein based (Biocare) as blocking agent for 10 minute(s) · Concentration: 000% · Temperature: 25°C
Username

Mrs. Julia Abramowitz

Verified customer

Submitted Oct 30 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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