Overview

  • Product nameAnti-HB EGF antibody [4G10]
    See all HB EGF primary antibodies
  • Description
    Mouse monoclonal [4G10] to HB EGF
  • Tested applicationsSuitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Recombinant human HB EGF ectodomain expressed in SF21 cells.

  • EpitopeEGF domain
  • Positive control
    • Vero cell extract carrrying human HB-EGF expression vector.

Properties

Applications

Our Abpromise guarantee covers the use of ab66792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa).
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • FunctionMay be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
  • Sequence similaritiesContains 1 EGF-like domain.
  • Post-translational
    modifications
    Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
    O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
  • Cellular localizationCell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
    • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
    • Diphtheria toxin receptor antibody
    • DT R antibody
    • DT-R antibody
    • DTR antibody
    • DTS antibody
    • DTSF antibody
    • HB-EGF antibody
    • HBEGF antibody
    • HBEGF_HUMAN antibody
    • HEGFL antibody
    • Heparin binding EGF like growth factor antibody
    • Heparin binding epidermal growth factor antibody
    • Heparin binding epidermal growth factor like growth factor antibody
    • Heparin-binding EGF-like growth factor antibody
    • Proheparin binding EGF like growth factor antibody
    see all

Anti-HB EGF antibody [4G10] images

  • All lanes : Anti-HB EGF antibody [4G10] (ab66792) at 1 µg/ml

    Lane 1 : Vero cell extract
    Lane 2 : Vero cell extract from cells carrrying human HB-EGF expression vector.
    Lane 3 : Vero cell extract from cells carrying mouse HB-EGF expression vector


    Predicted band size : 23 kDa
    Observed band size : 20-28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-HB EGF antibody [4G10] (ab66792) at 1 µg/ml

    Lane 1 : Vero cell extract
    Lane 2 : Vero cell extract from cells carrrying human HB-EGF expression vector.
    Lane 3 : Vero cell extract from cells carrying mouse HB-EGF expression vector


    Predicted band size : 23 kDa
    Observed band size : 20-38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
  • These images show Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HB-EGF expression vector (right hand image). Cells treated with ab 66792, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.

References for Anti-HB EGF antibody [4G10] (ab66792)

This product has been referenced in:
  • Tang XH  et al. The anti-tumor effect of cross-reacting material 197, an inhibitor of heparin-binding EGF-like growth factor, in human resistant ovarian cancer. Biochem Biophys Res Commun 422:676-80 (2012). Human . Read more (PubMed: 22609777) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"