Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)
Key features and details
- Biotin Mouse monoclonal [4G10] to HBEGF/DTR
- Suitable for: IP, ICC/IF, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Biotin Anti-HBEGF/DTR antibody [4G10]
See all HBEGF/DTR primary antibodies -
Description
Biotin Mouse monoclonal [4G10] to HBEGF/DTR -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse -
Immunogen
Recombinant fragment corresponding to Human HBEGF/DTR.
Database link: Q99075 -
Epitope
EGF domain -
Positive control
- Vero cell extract carrying human HG-EGF expression vector.
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General notes
Biotin/IgG = 7.5
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Produced in serum-free medium and purified by combination of chromatography. -
Clonality
Monoclonal -
Clone number
4G10 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent dilution.
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ICC/IF |
Use at an assay dependent dilution.
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WB |
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa).
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Notes |
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IP
Use at an assay dependent dilution. |
ICC/IF
Use at an assay dependent dilution. |
WB
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. -
Sequence similarities
Contains 1 EGF-like domain. -
Post-translational
modificationsSeveral N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937). -
Cellular localization
Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor. - Information by UniProt
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Database links
- Entrez Gene: 1839 Human
- Omim: 126150 Human
- SwissProt: Q99075 Human
- Unigene: 799 Human
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Alternative names
- diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
- diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
- Diphtheria toxin receptor antibody
see all
Images
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All lanes : Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793) at 1 µg/ml
Lane 1 : Vero (African green monkey kidney epithelial cell) whole cell lysate
Lane 2 : Vero cell extract from cells carrying human HBEGF/DTR expression vector
Lane 3 : Vero cell extract from cells carrying mouse HBEGF/DTR expression vector
Predicted band size: 23 kDa
Observed band size: 20-28 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands. -
These images shows Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR expression vector (right hand image). Cells treated with ab66793, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
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All lanes : HBEGF/DTR antibody [4G10](biotin) - Azide free (ab66793) at 1 µg/ml. Lane 1 : Vero cell extract Lane 2 : Vero cell extract from cells carrrying human HBEGF/DTR expression vector. Lane 3 : Vero cell extract from cells carrying mouse HBEGF/DTR expression vector Predicted band size : 23 kDa Observed band size : 20-38 kDa Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands. This image shows Immunoprecipitation of HBEGF/DTR followed by Western blotting/SDS PAGE; the cell surface was biotinylated.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab66793 has not yet been referenced specifically in any publications.