Anti-HBEGF/DTR antibody [MM0324-3R44] (ab89241)
Key features and details
- Mouse monoclonal [MM0324-3R44] to HBEGF/DTR
- Suitable for: Neutralising, WB
- Reacts with: Human
- Isotype: IgG2
Overview
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Product name
Anti-HBEGF/DTR antibody [MM0324-3R44]
See all HBEGF/DTR primary antibodies -
Description
Mouse monoclonal [MM0324-3R44] to HBEGF/DTR -
Host species
Mouse -
Tested applications
Suitable for: Neutralising, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human HBEGF/DTR.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography. -
Clonality
Monoclonal -
Clone number
MM0324-3R44 -
Isotype
IgG2 -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Neutralising |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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Neutralising
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. -
Sequence similarities
Contains 1 EGF-like domain. -
Post-translational
modificationsSeveral N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937). -
Cellular localization
Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor. - Information by UniProt
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Database links
- Entrez Gene: 1839 Human
- Omim: 126150 Human
- SwissProt: Q99075 Human
- Unigene: 799 Human
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Alternative names
- diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
- diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
- Diphtheria toxin receptor antibody
see all
Datasheets and documents
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Datasheet download
References (2)
ab89241 has been referenced in 2 publications.
- Luo P et al. s-HBEGF/SIRT1 circuit-dictated crosstalk between vascular endothelial cells and keratinocytes mediates sorafenib-induced hand-foot skin reaction that can be reversed by nicotinamide. Cell Res 30:779-793 (2020). PubMed: 32296111
- Liu J et al. CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17. Int J Biol Sci 15:493-506 (2019). PubMed: 30745837