Overview

  • Product nameAnti-HB9/HLXB9 antibodySee all HB9/HLXB9 primary antibodies ...
  • Description
    Sheep polyclonal to HB9/HLXB9
  • Specificityab59795 is specific for for HB9/HLXB9.
  • Tested applicationsIHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    H-APADRLRAESPSPPRLLAAHC-NH2

    , corresponding to amino acids 68-88 of Human HB9/HLXB9 conjugated to Blue Carrier Protein.

  • Positive control
    • Normal Term and First Trimester human Placenta and mouse Neuroblastoma and Spinal Cord cells.
  • General notesFor additional stability glycerol at a concentration of 1:1 can be added.

Properties

Applications

Our Abpromise guarantee covers the use of ab59795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 41 kDa.
ELISA 1/500 - 1/6500.

Target

  • FunctionPutative transcription factor involved in pancreas development and function.
  • Tissue specificityExpressed in lymphoid and pancreatic tissues.
  • Involvement in diseaseDefects in MNX1 are a cause of Currarino syndrome (CURRAS) [MIM:176450]. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HB 9 antibody
    • HB9 antibody
    • HLXB 9 antibody
    • HLXB9 antibody
    • Homeo box HB9 antibody
    • Homeobox HB9 antibody
    • Homeobox protein HB9 antibody
    • HOXHB9 antibody
    • MNX1 antibody
    • MNX1_HUMAN antibody
    • Motor neuron and pancreas homeobox protein 1 antibody
    • Sacral agenesis autosomal dominant (Currarino triad) antibody
    • SCRA 1 antibody
    • SCRA1 antibody
    see all

References for Anti-HB9/HLXB9 antibody (ab59795)

This product has been referenced in:
  • Zoldan J  et al. The influence of scaffold elasticity on germ layer specification of human embryonic stem cells. Biomaterials 32:9612-21 (2011). IHC-P ; Human . Read more (PubMed: 21963156) »
  • Neufing PJ  et al. Expression and localization of homeodomain proteins DLX4/HB9 in normal and malignant human breast tissues. Anticancer Res 23:1479-88 (2003). IHC-P ; Human . Read more (PubMed: 12820413) »

See all 2 Publications for this product

Product Wall

I have found a paper describing development of the HB9 antibody ab59795 and the protocol that was used for IHC on tumor tissue.

Neufing PJ et al. Expression and localization of homeodomain proteins DLX4/HB9 in normal and malignant human bre...

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Thank you for the reassurance. If the IHC continues to be a problem, please contact me. Good luck!

I will send the IHC protocol for ab59795 when I receive it, hoepfully in the next day or two. As I mentioned, Abcam does not validate this antibody for IHC in-house. We do not have free samples of any of our antibodies, but ab59795 is covered by our Ab...

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Thank you for sending the details of your protocol. I agree that the staining you see for both of these antibodies is not what is expected. I think what you want is shown on the datasheet for a different antibody against HB9, ab92606, showing a stain o...

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Thank you for bringing this to our attention. Can you please send a few more details of your protocol? In particular, I would like to know if you included an antigen retrieval step. Also, did you confirm that the secondary is not the issue, by leaving ...

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