Overview

  • Product name
  • Description
    Rabbit polyclonal to HB9/HLXB9
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide, corresponding to a portion of amino acids 330 - 380 of Mouse HB9/HLXB9 (NP_064328.2).

  • Positive control
    • WB: MOLT 4 cell lysate; IHC-P: Mouse pancreas tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab92606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    IHC-P: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 0.5 - 2.0 µg/ml. Predicted molecular weight: 41 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Putative transcription factor involved in pancreas development and function.
    • Tissue specificity
      Expressed in lymphoid and pancreatic tissues.
    • Involvement in disease
      Defects in MNX1 are a cause of Currarino syndrome (CURRAS) [MIM:176450]. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait.
    • Sequence similarities
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • HB 9 antibody
      • HB9 antibody
      • HLXB 9 antibody
      • HLXB9 antibody
      • Homeo box HB9 antibody
      • Homeobox HB9 antibody
      • Homeobox protein HB9 antibody
      • HOXHB9 antibody
      • MNX1 antibody
      • MNX1_HUMAN antibody
      • Motor neuron and pancreas homeobox 1 antibody
      • Motor neuron and pancreas homeobox protein 1 antibody
      • Sacral agenesis autosomal dominant (Currarino triad) antibody
      • SCRA 1 antibody
      • SCRA1 antibody
      see all

    Images

    • Immunohistochemistry analysis of HB9/HLXB9 expression in formalin-fixed, paraffin-embedded Mouse pancreas tissue using: an isotype control (1) or ab92606 at 5µg/ml (2 and 3).
    • All lanes : Anti-HB9/HLXB9 antibody (ab92606) at 0.5 µg/ml

      Lane 1 : MOLT 4 cell lysate
      Lane 2 : MOLT 4 cell lysate with immunizing peptide

      Secondary
      HRP-conjugated Goat anti-Rabbit IgG

      Predicted band size : 41 kDa

    References

    This product has been referenced in:
    • Serre A  et al. Overexpression of basic helix-loop-helix transcription factors enhances neuronal differentiation of fetal human neural progenitor cells in various ways. Stem Cells Dev 21:539-53 (2012). Human . Read more (PubMed: 21561385) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (e10.5 embryonic mouse spinal cord)
    Specification
    e10.5 embryonic mouse spinal cord
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Dr. Frederic Clotman

    Verified customer

    Submitted Sep 13 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (spinal cord culture)
    Specification
    spinal cord culture
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.3% Triton X100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 28 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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