Recombinant
RabMAb

Anti-HB9/HLXB9 antibody [EPR3342] (ab79541)

Overview

  • Product name
    Anti-HB9/HLXB9 antibody [EPR3342]
    See all HB9/HLXB9 primary antibodies
  • Description
    Rabbit monoclonal [EPR3342] to HB9/HLXB9
  • Specificity
    There have been conflicting results with this antibody in IHC-P. PMID 21484430 reported specific labeling in IHC-P, but internally we were unable to reproduce these results. Thus, we have removed IHC-P as a guaranteed application, and we welcome any further feedback from customers using this antibody in IHC-P.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminal of Human HB9/HLXB9.

  • Positive control
    • Molt-4 and Raji cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab79541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
IP 1/50.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Putative transcription factor involved in pancreas development and function.
    • Tissue specificity
      Expressed in lymphoid and pancreatic tissues.
    • Involvement in disease
      Defects in MNX1 are a cause of Currarino syndrome (CURRAS) [MIM:176450]. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait.
    • Sequence similarities
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • HB 9 antibody
      • HB9 antibody
      • HLXB 9 antibody
      • HLXB9 antibody
      • Homeo box HB9 antibody
      • Homeobox HB9 antibody
      • Homeobox protein HB9 antibody
      • HOXHB9 antibody
      • MNX1 antibody
      • MNX1_HUMAN antibody
      • Motor neuron and pancreas homeobox 1 antibody
      • Motor neuron and pancreas homeobox protein 1 antibody
      • Sacral agenesis autosomal dominant (Currarino triad) antibody
      • SCRA 1 antibody
      • SCRA1 antibody
      see all

    Images

    • All lanes : Anti-HB9/HLXB9 antibody [EPR3342] (ab79541) at 1/200000 dilution

      Lane 1 : Molt-4 cell lysate
      Lane 2 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 41 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Egawa N  et al. Drug screening for ALS using patient-specific induced pluripotent stem cells. Sci Transl Med 4:145ra104 (2012). Human . Read more (PubMed: 22855461) »
    • Wilkens L  et al. The homeobox gene HLXB9 is upregulated in a morphological subset of poorly differentiated hepatocellular carcinoma. Virchows Arch 458:697-708 (2011). WB, IHC-P ; Human . Read more (PubMed: 21484430) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    I will send the IHC protocol for ab59795 when I receive it, hoepfully in the next day or two. As I mentioned, Abcam does not validate this antibody for IHC in-house. We do not have free samples of any of our antibodies, but ab59795 is covered by our Ab...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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