Anti-HBP1 (phospho S402) antibody (ab73339)

Overview

  • Product name
    Anti-HBP1 (phospho S402) antibody
    See all HBP1 primary antibodies
  • Description
    Rabbit polyclonal to HBP1 (phospho S402)
  • Specificity
    ab73339 detects endogenous levels of HBP1 only when phosphorylated at serine 402 (Human) or serine 404 (Mouse).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human HBP1 around the phosphorylation site of serine 402 (CGSPPG)

  • Positive control
    • Extracts from A549 cells treated with PMA (125ng/ml, 30mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab73339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
ELISA 1/5000.

Target

  • Function
    Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.
  • Sequence similarities
    Contains 1 AXH domain.
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ16340 antibody
    • HBP 1 antibody
    • HBP1 antibody
    • HBP1_HUMAN antibody
    • High mobility group box transcription factor 1 antibody
    • HMG box containing protein 1 antibody
    • HMG box transcription factor 1 antibody
    • HMG box-containing protein 1 antibody
    see all

Images

  • All lanes : Anti-HBP1 (phospho S402) antibody (ab73339) at 1/500 dilution

    Lane 1 : Extracts from A549 cells treated with PMA (125ng/ml, 30 mins)
    Lane 2 : Extracts from A549 cells treated with PMA (125ng/ml, 30 mins) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 58 kDa
    Observed band size : 58 kDa

References

ab73339 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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