Overview

  • Product name
  • Description
    Rabbit polyclonal to HBZ
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Cow, Pig, Chimpanzee
  • Immunogen

    A region within synthetic peptide: HPQTKTYFPH FDLHPGSAQL RAHGSKVVAA VGDAVKSIDD IGGALSKLSE, corresponding to amino acids 37-86 of Human HBZ

  • Positive control
    • K562 cell lysate. Human liver.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

Images

  • All lanes : HBZ antibody (ab62266) at 1.25µg/ml in 5% skim milk / PBS buffer

    Lane 1 : Molecular weight marker
    Lane 2 : K562 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 15%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelled HBZ with ab62266 at 4-8µg/ml. Arrows indicate positively labelled hemopoietic cells. Magnification: 400X.

References

ab62266 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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