Overview

  • Product name
  • Description
    Goat polyclonal to HCCA2
  • Specificity
    ab115013 is expected to recognize both reported isoforms (NP_001165694.1; NP_443731.2) only.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    DERGKKVKCTAPQ

    , corresponding to internal sequence amino acids 128-140 of Human HCCA2 (NP_001165694.1; NP_443731.2).

  • Positive control
    • Jurkat cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab115013 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.02 - 0.06 µg/ml. Detects a band of approximately 30, 48 kDa (predicted molecular weight: 30 kDa). An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide.

Target

Images

  • ab115013 (5µg/ml) shows staining of HCCA2 in paraffin embedded Human Lung following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole.

  • Anti-HCCA2 antibody (ab115013) at 0.02 µg/ml + Jurkat cell lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 30 kDa

References

ab115013 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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