Overview

  • Product name
  • Description
    Rabbit polyclonal to HCE
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human HCE aa 1-50. The exact sequence is proprietary. NP_003791.3
    Sequence:

    MAHNKIPPRW LNCPRRGQPV AGRFLPLKTM LGPRYDSQVA EENRFHPSML


    Database link: O60942

  • Positive control
    • HeLa, 293T and NIH3T3 cell lysates, Human ovarian carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab176728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/250.

Target

  • Function
    Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
  • Tissue specificity
    Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle.
  • Sequence similarities
    In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.
    In the C-terminal section; belongs to the eukaryotic GTase family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAP1A antibody
    • GTase antibody
    • GTP--RNA guanylyltransferase antibody
    • hCAP antibody
    • HCAP1 antibody
    • HcE antibody
    • HCE1 antibody
    • MCE1 antibody
    • MCE1_HUMAN antibody
    • mRNA 5''-triphosphatase antibody
    • mRNA capping enzyme antibody
    • mRNA guanyltransferase antibody
    • mRNA guanylyltransferase antibody
    • Polynucleotide 5' trisphosphatase antibody
    • RNA guanylyltransferase and 5' phosphatase antibody
    • RNGTT antibody
    • TPase antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human ovarian carcinoma tissue labeling HCE using ab176728 at a 1/200 dilution (1µg/ml), using peroxidase substrate for detection.

  • Western blot of immunopricipitate of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), labeling HCE using ab176728 for WB at 0.4 µg/ml and used for IP at 3 µg/mg lysate.

  • All lanes : Anti-HCE antibody (ab176728) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 69 kDa


    Exposure time: 3 minutes

References

ab176728 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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