Overview

  • Product nameAnti-HCE antibody
    See all HCE primary antibodies
  • Description
    Sheep polyclonal to HCE
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) with 6x His tag

Properties

Applications

Our Abpromise guarantee covers the use of ab27971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP Use at an assay dependent dilution.

Target

  • FunctionBifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
  • Tissue specificityIsoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle.
  • Sequence similaritiesIn the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.
    In the C-terminal section; belongs to the eukaryotic GTase family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAP1A antibody
    • GTase antibody
    • GTP--RNA guanylyltransferase antibody
    • hCAP antibody
    • HCAP1 antibody
    • HcE antibody
    • HCE1 antibody
    • MCE1 antibody
    • MCE1_HUMAN antibody
    • mRNA 5''-triphosphatase antibody
    • mRNA capping enzyme antibody
    • mRNA guanyltransferase antibody
    • mRNA guanylyltransferase antibody
    • Polynucleotide 5' trisphosphatase antibody
    • RNA guanylyltransferase and 5' phosphatase antibody
    • RNGTT antibody
    • TPase antibody
    see all

Anti-HCE antibody images

  • All lanes : Anti-HCE antibody (ab27971) at 1/1000 dilution

    Lane 1 : Hela nuclear extract at 0.5 µl
    Lane 2 : Hela nuclear extract at 1 µl

    Secondary
    donkey anti-sheep peroxidase conjugate at 1/20000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa

References for Anti-HCE antibody (ab27971)

This product has been referenced in:
  • Adamson TE  et al. Functional coupling of cleavage and polyadenylation with transcription of mRNA. J Biol Chem 280:32262-71 (2005). Read more (PubMed: 16041059) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"