Anti-HCF-1 / Host Cell Factor C1 antibody (ab94631)

Overview

  • Product nameAnti-HCF-1 / Host Cell Factor C1 antibody
    See all HCF-1 / Host Cell Factor C1 primary antibodies
  • Description
    Rabbit polyclonal to HCF-1 / Host Cell Factor C1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 1986-2035 (RNEKGYGPAT QVRWLQETSK DSSGTKPANK RPMSSPEMKS APKKSKADGQ) of Human Host Cell Factor C1 (NP_005325).

  • Positive control
    • Human fetal stomach lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 209 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInvolved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes.
  • Tissue specificityHighly expressed in fetal tissues and the adult kidney. Present in all tissues tested.
  • Sequence similaritiesContains 5 Kelch repeats.
  • DomainThe HCF repeat is a highly specific proteolytic cleavage signal.
    The kelch repeats fold into a 6-bladed kelch beta-propeller called the beta-propeller domain which mediates interaction with HCFC1R1.
  • Post-translational
    modifications
    Proteolytically cleaved at one or several PPCE--THET sites within the HCF repeats. Further cleavage of the primary N- and C-terminal chains results in a 'trimming' and accumulation of the smaller chains.
    O-glycosylated.
    Ubiquitinated. Lys-1807 and Lys-1808 are ubiquitinated both via 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. BAP1 mediated deubiquitination of 'Lys-48'-linked polyubiquitin chains; deubiquitination by BAP1 does not seem to stabilize the protein.
  • Cellular localizationCytoplasm. Nucleus. HCFC1R1 modulates its subcellular localization and overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm. Nuclear in general, but uniquely cytoplasmic in trigeminal ganglia, becoming nuclear upon HSV reactivation from the latent state.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1 factor antibody
    • CFF antibody
    • HCF 1 antibody
    • HCF antibody
    • HCF C-terminal chain 6 antibody
    • HCF-1 antibody
    • HCF1 antibody
    • Hcfc1 antibody
    • HCFC1_HUMAN antibody
    • HFC1 antibody
    • Host cell factor 1 antibody
    • Host cell factor antibody
    • Host cell factor C1 (VP16 accessory protein) antibody
    • Host cell factor C1 antibody
    • MGC70925 antibody
    • MRX3 antibody
    • VCAF antibody
    • VP16 accessory protein antibody
    see all

Anti-HCF-1 / Host Cell Factor C1 antibody images



  • Predicted band size : 209 kDa
    Western blot analysis of HIS-HCFC1 16-363aa (lane 1 - 42kD) transformed bacteria lysate and GFP-HCFC1 363-2002aa (73kD - lane 2) transformed bacteria lysate elution sample labeling HCF-1 / Host Cell Factor C1 with ab94631 at 1:1000. An Alexa Fluor 680-conjugated anti-rabbit (1:10000) was used as the secondary antibody.


  • Predicted band size : 209 kDa
    Western blot analysis of Human fetal liver tissue lsyate labeling HCF-1 / Host Cell Factor C1 with ab94631 at 1.0µg/ml.


  • Predicted band size : 209 kDa
    Western blot analysis of Human fetal heart tissue lsyate labeling HCF-1 / Host Cell Factor C1 with ab94631 at 1.0µg/ml.
  • Anti-HCF-1 / Host Cell Factor C1 antibody (ab94631) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal stomach lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 209 kDa
    Gel concentration: 6-18%

References for Anti-HCF-1 / Host Cell Factor C1 antibody (ab94631)

ab94631 has not yet been referenced specifically in any publications.

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