Anti-hCG alpha 4 antibody [INN-hFSH-132] (ab11232)

Overview

  • Product nameAnti-hCG alpha 4 antibody [INN-hFSH-132]
  • Description
    Mouse monoclonal [INN-hFSH-132] to hCG alpha 4
  • SpecificityA high affinity antibody recognising the alpha 4 epitope of the alpha-subunit of Human Chorionic Gonadotrophin, hLH, hFSH, hTSH and free alpha-subunit. The recognised epitope comprises amino-acids alpha 15 to alpha 22 of the human sequence. We have data to indicate that this antibody may not cross react with wide range of other species. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: IHC-P, ELISA, IP, RIA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human Follicle Stimulating Hormone

  • General notesThis product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ELISA
IP
RIA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    IHC-P: Used at a dilution of 1/1500 for 2 hrs on human placenta sections (see Abreview for further details).
    IP: Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution (see Berger et al).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHuman chorionic gonadotropin (hCG) is a peptide hormone produced in pregnancy, by the developing placenta and by the fertilized egg after implantation in the uterine wall. Its primary role is in the maintenance of the corpus luteum and causes it to secrete the hormone progesterone. Progesterone enriches the uterus with a thick lining of blood vessels and capillaries so that it can sustain the growing fetus. HCG is composed of two subunits, alpha and beta. The alpha subunit of HCG, however, is identical to the alpha subunit of luteinising hormone, thyroid stimulating hormone and follicle stimulating hormone. Serum human chorionic gonadotrophin testing is accurate 48 hour post conception. Urine human chorionic gonadotrophin testing is a popular method of home pregnancy determination as human chorionic gonadotrophin can be detected in urine
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • CG alpha antibody
      • CGA antibody
      • FSH alpha antibody
      • Glycoprotein hormones alpha chain antibody
      • LSH alpha antibody
      • TSH alpha antibody
      see all

    References for Anti-hCG alpha 4 antibody [INN-hFSH-132] (ab11232)

    This product has been referenced in:
    • Adachi K  et al. Role of SOX2 in maintaining pluripotency of human embryonic stem cells. Genes Cells : (2010). Read more (PubMed: 20384793) »
    • Forbes K  et al. Insulin-like growth factor I and II regulate the life cycle of trophoblast in the developing human placenta. Am J Physiol Cell Physiol 294:C1313-22 (2008). IHC-P ; Human . Read more (PubMed: 18400990) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your reply and for your question.

    The lab has found that the volume is critical to the reaction, and theefficiency of the reaction will be significantly lower if more than 100 uL used. With 200 uL, I would be concerned that very...

    Read More

    Thank you for contacting us with your question.

    We do have abiotinylation kit thatis suitable to use with these detection antibodies, ab102864-

    http://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100µg-Biotin-ab102864.ht...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (placenta)
    Specification placenta
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
    Username

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    Verified customer

    Submitted Jul 17 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"