Anti-hCG antibody [28A4] (ab36473)


  • Product nameAnti-hCG antibody [28A4]
    See all hCG primary antibodies
  • Description
    Mouse monoclonal [28A4] to hCG
  • SpecificityThis antibody specifically recognizes the beta subunit of HCG and does not cross react with hLH, hTSH, hFSH.
  • Tested applicationsSuitable for: Sandwich ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human Placenta.
  • General notes

    This antibody specifically recognizes the beta subunit of HCG.


Associated products


Our Abpromise guarantee covers the use of ab36473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [27E8] to hCG (ab1961). Can be used in ELISA with ab1964 or ab1961 Best recommended pairs for sandwich immunoassay (coating - conjugate): ab1961 - ab36473 ab1964 - ab36473
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • RelevanceHuman chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • anterior pituitary glycoprotein hormones common subunit alpha antibody
    • CG-ALPHA antibody
    • CGB antibody
    • CGB3 antibody
    • CGB5 antibody
    • CGB7 antibody
    • choriogonadotropin alpha chain antibody
    • Chorionic gonadotrophin chain beta antibody
    • chorionic gonadotrophin subunit alpha antibody
    • chorionic gonadotropin beta 3 subunit antibody
    • Chorionic gonadotropin beta chain antibody
    • Chorionic gonadotropin beta subunit antibody
    • chorionic gonadotropin, alpha polypeptide antibody
    • chorionic gonadotropin, beta polypeptide 3 antibody
    • chorionic gonadotropin, beta polypeptide antibody
    • follicle-stimulating hormone alpha chain antibody
    • follicle-stimulating hormone alpha subunit antibody
    • follitropin alpha chain antibody
    • FSH-alpha antibody
    • FSHA antibody
    • glycoprotein hormones alpha chain antibody
    • glycoprotein hormones, alpha polypeptide antibody
    • GPHa antibody
    • GPHA1 antibody
    • HCG antibody
    • hCGB antibody
    • Human Chorionic Gonadotropin antibody
    • LHA antibody
    • LSH-alpha antibody
    • luteinizing hormone alpha chain antibody
    • lutropin alpha chain antibody
    • thyroid-stimulating hormone alpha chain antibody
    • thyrotropin alpha chain antibody
    • TSH-alpha antibody
    • TSHA antibody
    see all

Anti-hCG antibody [28A4] images

  • IHC image of hCG staining in Human Placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36473, 10 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-hCG antibody [28A4] (ab36473)

ab36473 has not yet been referenced specifically in any publications.

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Unfortunately the components of this kit are considered proprioetary by the lab and as such I do not have the information which you are requesting. However we do offer hCG antibody pairs which are tested and guaranteed in sELISA. The best recommended...

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