Anti-hCG antibody (ab54410)
Key features and details
- Rabbit polyclonal to hCG
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-hCG antibody
See all hCG primary antibodies -
Description
Rabbit polyclonal to hCG -
Host species
Rabbit -
Specificity
This antibody recognises both alpha and beta subunits of hCG. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified Chorionic Gonadotropin from human placental glucoprotein urine.
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Positive control
- Purified human CG and rat liver tissue lysate.
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 5778 Chorionic Gonadotropin (CG) Antibody. 5778-100 is the same size as the 100 µg size of ab54410.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.5% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab54410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 4 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 4 µg/ml. |
Target
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Relevance
Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 1081 Human
- Entrez Gene: 1082 Human
- Omim: 118850 Human
- Omim: 118860 Human
- SwissProt: P01215 Human
- SwissProt: P01233 Human
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Alternative names
- anterior pituitary glycoprotein hormones common subunit alpha antibody
- CG alpha antibody
- CG-ALPHA antibody
see all
Images
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All lanes : Anti-hCG antibody (ab54410) (0.5-4 ug/m)
Lane 1 : Recombinant Human Chorionic Gonadotropin at 0.01 µg
Lane 2 : Recombinant Human Chorionic Gonadotropin at 0.05 µg
Lane 3 : Recombinant Human Chorionic Gonadotropin at 0.25 µg
Lane 4 : Recombinant Human Chorionic Gonadotropin at 1 µgThis information is only intended as a guide. The optimal dilutions must be determined by the user.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab54410 has been referenced in 8 publications.
- Zhu H et al. NPFF increases fusogenic proteins syncytin 1 and syncytin 2 via GCM1 in first trimester primary human cytotrophoblast cells. FASEB J N/A:N/A (2020). PubMed: 32501590
- Hong G et al. New photothermal immunoassay of human chorionic gonadotropin using Prussian blue nanoparticle-based photothermal conversion. Anal Bioanal Chem 411:6837-6845 (2019). PubMed: 31471682
- Lu X et al. Twist1 is involved in trophoblast syncytialization by regulating GCM1. Placenta 39:45-54 (2016). PubMed: 26992674
- Li Y et al. Involvement of nephrin in human placental trophoblast syncytialization. Reproduction 149:339-46 (2015). PubMed: 25614620
- Zhou Z et al. The cAMP-responsive element binding protein (CREB) transcription factor regulates furin expression during human trophoblast syncytialization. Placenta 35:907-18 (2014). PubMed: 25175744
- Wang R et al. Live cell imaging of in vitro human trophoblast syncytialization. Biol Reprod 90:117 (2014). PubMed: 24740598
- Zhou Z et al. The proprotein convertase furin is required for trophoblast syncytialization. Cell Death Dis 4:e593 (2013). WB ; Human . PubMed: 23598405
- Dukic-Stefanovic S et al. Chorionic gonadotropin and its receptor are both expressed in human retina, possible implications in normal and pathological conditions. PLoS One 7:e52567 (2012). WB ; Human . PubMed: 23285091