• Product nameAnti-hCG beta antibody [5H4-E2]
    See all hCG beta primary antibodies
  • Description
    Mouse monoclonal [5H4-E2] to hCG beta
  • SpecificityThe antibody recognizes the whole hCG beta and the C-terminal end part of hCG beta, as well as the hCG dimer molecule. It does not react with hCG alpha, FSH, LH, TSH and albumins (evaluated by simple-binding ELISA using 1.00 µg/well purified antigens (WHO standards) and 1.00 µg/ml primary antibody concentrations). This antibody labels the cytoplasm of hCG beta producing (trophoblastic) cells in placenta, mola hydatidosa, choriocarcinoma, embryonal carcinoma and some undifferenciated lung cancers, as well as, the JAR choriocarcinoma cell line. It does not react with cells of normal endometrial, testicular tissues and lung (tested by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections using the Streptavidin-biotinylated peroxidase method).
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Horse
  • Immunogen

    Full length native protein (purified) (Human).



Our Abpromise guarantee covers the use of ab9582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. It gives optimal immunostaining with the majority of well-known detection systems (e.g. LSAB, ABC/SABC, PAP, APAAP, double staining, etc.). Antigen retrieval, such as heating of slides in 10 mM citrate buffer, pH 6.00 or proteolytic pre-treatment of tissues may increase the staining intensity. The recommended time of incubation is 60 minutes at room temperature.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.


Anti-hCG beta antibody [5H4-E2] images

  • ab9582 at 1/100 dilution staining hCG beta in human Chorionic villus cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, without permeabilization and blocked in 3% BSA for 1 hour at 25°C. The primary antibody was diluted in PBS (3%BSA) and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 568 conjugated Goat polyclonal to rabbit IgG was used at 1/500 dilution as secondary. Image A show DAPI staining, B show staining with ab9582 and C is the merged image which show staining with DAPI and ab9582 together. 

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  • ab9582 staining hCG beta in human fetal chorionic villus by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Samples were fixed in 4% paraformaldehyde and then incubated with ab9582 in PBT [3% bovine serum albumin (BSA) and 0.5% Triton X-100 in phosphate-buffered saline (PBS)] overnight at 4°C. After extensive washing with PBT, samples were incubated with a secondary antibody conjugated to Alexa-Fluor 488 for 1 hour at room temperature in the dark. After nuclear staining with Hoechst slides were mounted with PBS/ glycerol.

References for Anti-hCG beta antibody [5H4-E2] (ab9582)

This product has been referenced in:
  • Li W  et al. Role of IGF2BP3 in trophoblast cell invasion and migration. Cell Death Dis 5:e1025 (2014). WB, IHC-P ; Human . Read more (PubMed: 24457969) »
  • Li Y  et al. BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state. Development 140:3965-76 (2013). Human . Read more (PubMed: 24004950) »

See all 3 Publications for this product

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Thank you for contacting Abcam regarding ab9582.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check th...

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I regret to inform to that we have not tested it in our lab so I am afraid that we can't advise on the binding characteristics to PDMS (Polydimethylsiloxane) surface. As I have mentioned in my previous e-mail, ab9582 has not been tested in immunoprecip...

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Thank you for your enquiry and your interest in our products.

I can confirm that ab9582 (Anti-hCG beta antibody [5H4-E2]), has not been tested for IP yet, though the isotype has been determined (IgG1).

Normally, we would suggest usi...

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Thank you for your enquiries.

I can confirm that ab9582hCG beta antibody was raised against the beta subunit ofhCG only, and its reactivity was blocked by betasubunit of hCG, notthe alpha subunit. Therefore, we would not expect it to detect ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Chorionic villus)
Specification Chorionic villus
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Paola Spitalieri

Verified customer

Submitted Jan 19 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Horse Recombinant protein (culture broth)
Loading amount 2 µg
Specification culture broth
Blocking step Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5

Dr. Anna Ubach

Verified customer

Submitted Jun 19 2006

Thank you for your feedback regarding ab9582. You mention a band at a molecular weight similar to that of albumin, is this around 60-70KDa? I think the MW for albumin is about 66KDa. Could this band be explained by the fact the antibody recognizes th...

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Thank you for the information that you have provided. We have not had any other complaints concerning this antibody. Since you have not been able to obtain a signal with the new vial under the same conditions that you used with the previous lot, I woul...

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