The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Use at a concentration of 1 - 2 µg/ml for 30 minutes at RT. Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Use in WB is not optimum.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionStimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.
Sequence similaritiesBelongs to the glycoprotein hormones subunit beta family.
Developmental stageMade by the first trimester placenta.
ab80786, at 2µg/ml, staining hCG beta in human placenta by Immunohistochemistry using formalin-fixed paraffin-embedded tissue, followed by a peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of trophoblasts.
References for Anti-hCG beta antibody [CG04 + CG05] (ab80786)
has not yet been referenced specifically in any publications.
Publishing research using ab80786? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"