Anti-hCG beta antibody [HCG-61] (ab764)

Overview

  • Product nameAnti-hCG beta antibody [HCG-61]
    See all hCG beta primary antibodies
  • Description
    Mouse monoclonal [HCG-61] to hCG beta
  • SpecificityReacts with beta subunit of human chorionic gonadotropin with association constant 5.1 x 1010 l/mol. The following cross-reactivities (%) were determined by classic double-antibody radioimmunoassay using unlabelled hormones: b-hCG = 77 a-hCG = 1.3 hLH = 0.86 hFSH = < 0.5 hTSH = < 0.5
  • Tested applicationsSuitable for: ELISA, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 3 - 10 µg/ml.
ICC Use a concentration of 3 - 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

Anti-hCG beta antibody [HCG-61] images

  • IHC image of hCG beta staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab764, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-hCG beta antibody [HCG-61] (ab764)

ab764 has not yet been referenced specifically in any publications.

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