Overview

  • Product nameAnti-Hck antibody
    See all Hck primary antibodies
  • Description
    Rabbit polyclonal to Hck
  • SpecificityRecognizes human Hck at 59kD. Weakly recognizes a band at 85kD.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Chimpanzee
  • Immunogen

    Synthetic peptide:

    FLQVGGNTFSKTETS

    , corresponding to amino acids 29-43 of Human Hck

  • Positive control
    • WB: RIPA lysates from HUT-78 cells IP: RIPA lysates from THP-1 monocytic cells

Properties

Applications

Our Abpromise guarantee covers the use of ab32860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
IP Use at an assay dependent dilution. Reported to Immunoprecipitate Hck from 1-2 x 10 7 THP-1 monocytic cells lysed in RIPA.

Target

  • FunctionMay serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
  • Tissue specificityExpressed predominantly in cells of the myeloid and B-lymphoid lineages.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • DomainThe SH3 domain mediates binding to HIV-1 Nef.
  • Cellular localizationMembrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
  • Information by UniProt
  • Database links
  • FormThis protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names
    • Bmk antibody
    • Hck 1 antibody
    • Hck antibody
    • HCK_HUMAN antibody
    • Hemopoietic cell kinase antibody
    • JTK9 antibody
    • p59-HCK/p60-HCK antibody
    • p59Hck antibody
    • p59HCK/p60HCK antibody
    • p61Hck antibody
    • Tyrosine protein kinase HCK antibody
    • Tyrosine-protein kinase HCK antibody
    see all

References for Anti-Hck antibody (ab32860)

This product has been referenced in:
  • Zhu L  et al. Surgical monotherapy may be a suitable therapeutic strategy for advanced collecting (Bellini) duct carcinoma: A case report and literature review. Exp Ther Med 12:1181-1184 (2016). IHC-P ; Human . Read more (PubMed: 27446340) »
  • Xiao X  et al. c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution. Am J Physiol Endocrinol Metab 304:E145-59 (2013). WB ; Rat . Read more (PubMed: 23169788) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"