Anti-Hck antibody [EP2415Y] (ab75839)

Overview

  • Product name
    Anti-Hck antibody [EP2415Y]
    See all Hck primary antibodies
  • Description
    Rabbit monoclonal [EP2415Y] to Hck
  • Specificity
    This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request, please contact our Scientific Support for further information.
  • Tested applications
    Suitable for: WB, IP, ICC, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of human Hck

  • Positive control
    • TF-1 cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab75839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000 - 1/200000. Predicted molecular weight: 59 kDa.
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
    • Tissue specificity
      Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • Domain
      The SH3 domain mediates binding to HIV-1 Nef.
    • Cellular localization
      Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
    • Information by UniProt
    • Database links
    • Form
      This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • Alternative names
      • Bmk antibody
      • Hck 1 antibody
      • Hck antibody
      • HCK_HUMAN antibody
      • Hemopoietic cell kinase antibody
      • JTK9 antibody
      • p59-HCK/p60-HCK antibody
      • p59Hck antibody
      • p59HCK/p60HCK antibody
      • p61Hck antibody
      • Tyrosine protein kinase HCK antibody
      • Tyrosine-protein kinase HCK antibody
      see all

    Images

    • Anti-Hck antibody [EP2415Y] (ab75839) at 1/200000 dilution + TF-1 cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 59 kDa
      Observed band size : 59 kDa

    References

    ab75839 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    We have not tested ab75839 in-house for cross reactivity with LCK,YES, or BLK so we cannot say for certain whether this antibody would be suitable for detecting these targets. However, in our WB tests we have only observed a single band at ~ 60 KDa wh...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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