Anti-Hck (phospho Y410) antibody (ab61055)


  • Product nameAnti-Hck (phospho Y410) antibody
    See all Hck primary antibodies
  • Description
    Rabbit polyclonal to Hck (phospho Y410)
  • SpecificityDetects target only when phosphorylated at tyrosine 410.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide derived from human Hck around the phosphorylation site of tyrosine 410 (N-E-YP-T-A)

  • Positive control
    • HeLa cells


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab61055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.
ELISA 1/20000.


  • FunctionMay serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
  • Tissue specificityExpressed predominantly in cells of the myeloid and B-lymphoid lineages.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • DomainThe SH3 domain mediates binding to HIV-1 Nef.
  • Cellular localizationMembrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
  • Information by UniProt
  • Database links
  • FormThis protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names
    • Bmk antibody
    • Hck 1 antibody
    • Hck antibody
    • HCK_HUMAN antibody
    • Hemopoietic cell kinase antibody
    • JTK9 antibody
    • p59-HCK/p60-HCK antibody
    • p59Hck antibody
    • p59HCK/p60HCK antibody
    • p61Hck antibody
    • Tyrosine protein kinase HCK antibody
    • Tyrosine-protein kinase HCK antibody
    see all

Anti-Hck (phospho Y410) antibody images

  • ab61055 staining Hck (phospho Y410) in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% BSA for 1 hour; antigen retrieval was by heat mediation in trypsin. Samples were incubated with primary antibody (1/100) for 24 hours. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • 1/500-1/1000 ab61055 staining phosphorilated Hck on HeLa cells in the absence (left) or presence (right) of the immunizing phosphopeptide.

References for Anti-Hck (phospho Y410) antibody (ab61055)

ab61055 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Trypsin
Sample Mouse Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative 10% Formalin

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Submitted Oct 30 2013