Overview

  • Product nameAnti-HCLS1 antibody
    See all HCLS1 primary antibodies
  • Description
    Rabbit polyclonal to HCLS1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: QRWGAKTIEG SGRTEHINIH QLRNKVSEEH DVLRKKEMES GPKASHGYGG, corresponding to N terminal amino acids 36-85 of Human HCLS1

  • Positive control
    • WB: Jurkat cell lysate. IHC-P: Human kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab28725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionSubstrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
  • Tissue specificityExpressed only in tissues and cells of hematopoietic origin.
  • Sequence similaritiesContains 4 cortactin repeats.
    Contains 1 SH3 domain.
  • Developmental stageExpressed in early stage of myeloid and erythroid differentiation.
  • Post-translational
    modifications
    Phosphorylated by LYN; rapidly after cross-linking of surface IgM on B-cells.
  • Cellular localizationMembrane. Cytoplasm. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cortactin like antibody
    • CTTNL antibody
    • HCLS 1 antibody
    • Hcls1 antibody
    • HCLS1_HUMAN antibody
    • Hematopoietic cell specific Lyn substrate 1 antibody
    • Hematopoietic cell-specific LYN substrate 1 antibody
    • Hematopoietic lineage cell-specific protein antibody
    • HS 1 antibody
    • HS1 antibody
    • LckBP1 antibody
    • OTTHUMP00000215180 antibody
    • OTTHUMP00000215182 antibody
    • p75 antibody
    see all

Anti-HCLS1 antibody images

  • Anti-HCLS1 antibody (ab28725) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • Ab28725, at a concentration of 4 - 8 µg/ml, staining HCLS1 in paraffin embedded human kidney tissue by Immunohistochemistry. Cells with positive label (as indicated by the arrows) are epithelial cells of the renal tubule. Magnification: 400X.
  • Immunohistochemistry of Human Heart lysate tissue at an antibody concentration of 4-8µg/ml using anti-HCLS1 antibody (ab28725)

  • Immunohistochemistry of Human Stomach tissue at an antibody concentration of 4-8µg/ml using anti-HCLS1 antibody (ab28725)

References for Anti-HCLS1 antibody (ab28725)

ab28725 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The bands in molecular weight marker we used before are a little deviate from the expected locations, therefore the 60kD band is about 70kD according to our new calibrated marker. This product should detect a band at arou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"