Overview

  • Product name
    Anti-HCLS1 antibody - C-terminal
    See all HCLS1 primary antibodies
  • Description
    Rabbit polyclonal to HCLS1 - C-terminal
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 423-486 of Human HCLS1.

  • Positive control
    • Jurkat, HL60, NIH 3T3 and JC whole cell lysates; Human gastric epithelium lymphocytes.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 54 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function
    Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
  • Tissue specificity
    Expressed only in tissues and cells of hematopoietic origin.
  • Sequence similarities
    Contains 4 cortactin repeats.
    Contains 1 SH3 domain.
  • Developmental stage
    Expressed in early stage of myeloid and erythroid differentiation.
  • Post-translational
    modifications
    Phosphorylated by LYN; rapidly after cross-linking of surface IgM on B-cells.
  • Cellular localization
    Membrane. Cytoplasm. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cortactin like antibody
    • CTTNL antibody
    • HCLS 1 antibody
    • Hcls1 antibody
    • HCLS1_HUMAN antibody
    • Hematopoietic cell specific Lyn substrate 1 antibody
    • Hematopoietic cell-specific LYN substrate 1 antibody
    • Hematopoietic lineage cell-specific protein antibody
    • HS 1 antibody
    • HS1 antibody
    • LckBP1 antibody
    • OTTHUMP00000215180 antibody
    • OTTHUMP00000215182 antibody
    • p75 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human gastric epithelium lymphocytes labeling HCLS1 with ab151511 at 1/100 dilution.
  • All lanes : Anti-HCLS1 antibody - C-terminal (ab151511) at 1/5000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 54 kDa
  • All lanes : Anti-HCLS1 antibody - C-terminal (ab151511) at 1/10000 dilution

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : HL60 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 54 kDa

References

ab151511 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab151511.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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