HCLS1 overexpression 293T lysate (whole cell) (ab94153)

Overview

  • Product name
    HCLS1 overexpression 293T lysate (whole cell)
  • Description
    HCLS1 overexpression lysate
  • General notes
    ab94153 is a 293T cell transfected lysate in which Human HCLS1 has been transiently over-expressed using a pCMV-HCLS1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Developmental stage: Expressed in early stage of myeloid and erythroid differentiation. Function: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. PTM: Phosphorylated by LYN; rapidly after cross-linking of surface IgM on B-cells. Similarity: Contains 4 cortactin repeats. Contains 1 SH3 domain. Tissue specificity: Expressed only in tissues and cells of hematopoietic origin.

Applications

Our Abpromise guarantee covers the use of ab94153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94153 at 15µg/lane on an SDS PAGE gel.
  • All lanes : Anti-HCLS1 antibody (ab55090) at 1/500 dilution

    Lane 1 : HCLS1 overexpression 293T lysate (whole cell) (ab94153)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94153 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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