Overview

  • Product name
  • Description
    Sheep polyclonal to HCM
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) with 6x His tag

Properties

Applications

Our Abpromise guarantee covers the use of ab27973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).
IP Use at an assay dependent dilution.

Target

  • Function
    mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the methyltransferase superfamily. mRNA cap methyltransferase family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686H1252 antibody
    • EC 2.1.1.56 antibody
    • hcm1p antibody
    • hCMT1 antibody
    • HCMT1a antibody
    • hCMT1c antibody
    • hMet antibody
    • hypothetical protein KIAA0396 antibody
    • KIAA0398 antibody
    • MCES_HUMAN antibody
    • MET antibody
    • mRNA (guanine 7)methyltransferase antibody
    • mRNA (guanine N(7)) methyltransferase antibody
    • mRNA (guanine-N(7)-)-methyltransferase antibody
    • mRNA cap guanine-N7 methyltransferase antibody
    • mRNA cap methyltransferase antibody
    • OTTHUMP00000162509 antibody
    • RG7MT1 antibody
    • RNA (guanine 7) methyltransferase antibody
    • RNA guanine 7 methyltransferase antibody
    • Rnmt antibody
    see all

Anti-HCM antibody images

  • All lanes : Anti-HCM antibody (ab27973) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HEK-293

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-sheep IgG HRP conjugate polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-HCM antibody (ab27973) at 1/1000 dilution

    Lane 1 : Recombinant 6xHis-tagged HCM
    Lane 2 : HeLa cell nuclear lysate at 0.25 µl
    Lane 3 : HeLa cell nuclear lysate at 0.5 µl

    Secondary
    donkey anti-sheep peroxidase linked at 1/20000 dilution

    Predicted band size : 55 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-HCM antibody (ab27973)

ab27973 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
20 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (12 % SDS PAGE gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted May 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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