Overview

  • Product nameAnti-HCM antibody
    See all HCM primary antibodies
  • Description
    Rabbit polyclonal to HCM
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide: LIRRLEASET ESFGNEIYTV KFQKKGDYPL FGCKYDFNLE GVVDVPEFLV , corresponding to C terminal amino acids 325-374 of Human HCM

  • Positive control
    • NTERA2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • FunctionmRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the methyltransferase superfamily. mRNA cap methyltransferase family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686H1252 antibody
    • EC 2.1.1.56 antibody
    • hcm1p antibody
    • hCMT1 antibody
    • HCMT1a antibody
    • hCMT1c antibody
    • hMet antibody
    • hypothetical protein KIAA0396 antibody
    • KIAA0398 antibody
    • MCES_HUMAN antibody
    • MET antibody
    • mRNA (guanine 7)methyltransferase antibody
    • mRNA (guanine N(7)) methyltransferase antibody
    • mRNA (guanine-N(7)-)-methyltransferase antibody
    • mRNA cap guanine-N7 methyltransferase antibody
    • mRNA cap methyltransferase antibody
    • OTTHUMP00000162509 antibody
    • RG7MT1 antibody
    • RNA (guanine 7) methyltransferase antibody
    • RNA guanine 7 methyltransferase antibody
    • Rnmt antibody
    see all

Anti-HCM antibody images

  • Anti-HCM antibody (ab80337) at 1 µg/ml + NTERA2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa

References for Anti-HCM antibody (ab80337)

ab80337 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"