Overview

  • Product nameAnti-HCN1 antibody
    See all HCN1 primary antibodies
  • Description
    Rabbit polyclonal to HCN1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit
  • Immunogen

    Synthetic peptide:

    LNTDPDAEKPRFASNL

    , corresponding to amino acids 875 - 890 of HCN1.

  • Positive control
    • Rat brain extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab19405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500. Detects a band of approximately 98, 115 kDa (predicted molecular weight: 98 kDa).

Target

  • FunctionHyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli.
  • Tissue specificityDetected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.
  • Sequence similaritiesBelongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.
  • DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCNG-1 antibody
    • BCNG1 antibody
    • Brain cyclic nucleotide gated channel 1 antibody
    • Brain cyclic nucleotide-gated channel 1 antibody
    • HAC 2 antibody
    • HAC2 antibody
    • HCN1 antibody
    • HCN1_HUMAN antibody
    • Hyperpolarization activated cyclic nucleotide gated potassium channel 1 antibody
    • Potassium channel, voltage-gated, brain, 1 antibody
    • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 antibody
    see all

Anti-HCN1 antibody images

  • IHC image of ab19405 staining in Human hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19405, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-HCN1 antibody (ab19405)

This product has been referenced in:
  • Atkinson SE & Williams SR Postnatal development of dendritic synaptic integration in rat neocortical pyramidal neurons. J Neurophysiol 102:735-51 (2009). Read more (PubMed: 19458150) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. It is not known whether ab19405 exactly recognizes an intra- or extracellular epitope, and so I would recommend trying a permeabilization step. The antibody was generated using a synthetic peptide: LNTDPDAEKPRFASNL, correspo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"