Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab19346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 100,115 kDa.

Target

  • FunctionHyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages.
  • Tissue specificityHighly expressed throughout the brain. Detected at low levels in heart.
  • Sequence similaritiesBelongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.
  • DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCNG-2 antibody
    • BCNG2 antibody
    • Brain cyclic nucleotide gated channel 2 antibody
    • Brain cyclic nucleotide-gated channel 2 antibody
    • HAC1 antibody
    • Hcn2 antibody
    • HCN2_HUMAN antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 antibody
    see all

Anti-HCN2 antibody images

  • ab19346 has been successfully used in Western blot procedures. By Western blot, this antibody detects bands at ~100 kDa and ~115 kDa representing the non-glycosylated and glycosylated form of HCN2 in rat brain extract.

References for Anti-HCN2 antibody (ab19346)

This product has been referenced in:
  • Kretschmannova K  et al. The expression and role of hyperpolarization-activated and cyclic nucleotide-gated channels in endocrine anterior pituitary cells. Mol Endocrinol 26:153-64 (2012). WB ; Rat . Read more (PubMed: 22135067) »
  • Adams BE  et al. Excitotoxic-mediated transcriptional decreases in HCN2 channel function increase network excitability in CA1. Exp Neurol 219:249-57 (2009). WB . Read more (PubMed: 19500574) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry. Unfortunately we do not sell the immunising peptide for this antibody. The Western blot protocol used to test this antibody can be found under our Protocol tab. Regarding lysate prep, you should be able to use a standard lys...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"