Anti-HCN2 antibody [S71-37] (ab84817)

Overview

  • Product nameAnti-HCN2 antibody [S71-37]
    See all HCN2 primary antibodies
  • Description
    Mouse monoclonal [S71-37] to HCN2
  • SpecificityDoes not cross react with HCN1.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein, corresponding to cytoplasmic C terminal amino acids 761-863 of Rat HCN2

  • Positive control
    • Rat brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 95 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent concentration.

Target

  • FunctionHyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages.
  • Tissue specificityHighly expressed throughout the brain. Detected at low levels in heart.
  • Sequence similaritiesBelongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.
  • DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCNG-2 antibody
    • BCNG2 antibody
    • Brain cyclic nucleotide gated channel 2 antibody
    • Brain cyclic nucleotide-gated channel 2 antibody
    • HAC1 antibody
    • Hcn2 antibody
    • HCN2_HUMAN antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 antibody
    see all

Anti-HCN2 antibody [S71-37] images

  • Anti-HCN2 antibody [S71-37] (ab84817) at 1/1000 dilution + Rat brain membrane lysate

    Predicted band size : 95 kDa
  • ab84817 staining HCN2 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

  • ab84817 staining HCN2 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).

References for Anti-HCN2 antibody [S71-37] (ab84817)

ab84817 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (HL-1 cardiac cell line)
Specification HL-1 cardiac cell line
Fixative Methanol
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Submitted Nov 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"