Recombinant
RabMAb

Anti-HCN3 [EPR13494(2)] antibody (ab192025)

Overview

  • Product name
    Anti-HCN3 [EPR13494(2)] antibody
    See all HCN3 primary antibodies
  • Description
    Rabbit monoclonal [EPR13494(2)] to HCN3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human HCN3 aa 650 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9P1Z3

  • Positive control
    • Human cerebellum, fetal brain, forebrain and hypothalamus lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
IP 1/30.

Target

  • Function
    Putative hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions.
  • Sequence similarities
    Belongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.
  • Domain
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hcn3 antibody
    • HCN3_HUMAN antibody
    • hyperpolarization activated cyclic nucleotide-gated potassium channel 3 antibody
    • KIAA1535 antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 antibody
    see all

Images

  • All lanes : Anti-HCN3 [EPR13494(2)] antibody (ab192025) at 1/10000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa
    Observed band size: 90 kDa (why is the actual band size different from the predicted?)



    Blocking/dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-HCN3 [EPR13494(2)] antibody (ab192025) at 1/1000 dilution

    Lane 1 : Human forebrain lysate
    Lane 2 : Human hypothalamus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa
    Observed band size: 90 kDa (why is the actual band size different from the predicted?)



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot analysis of HCN3 in Human fetal brain lysate immunoprecipitated using ab192025 at 1/30 dilution.

    Secondary antibody:  Goat anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution. 

    Blocking/dilution buffer: 5% NFDM/TBST.

References

ab192025 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192025.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up