Overview

  • Product name
  • Description
    Rabbit polyclonal to HCN4
  • Host species
    Rabbit
  • Specificity
    Reacts with human HCN4. Predicted to cross react with HCN4 proteins from mouse, rat and other species.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human HCN4 (N terminal).
    Database link: Q9Y3Q4

  • Positive control
    • ICC/IF: SKNSH cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab66501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/5000. Predicted molecular weight: 134 kDa.
ICC/IF 1/1000.

Target

  • Relevance
    HCN4 is a member of the family of hyperpolarization activated and cyclic nucleotide gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.
  • Cellular localization
    Membrane; multi pass membrane protein.
  • Database links
  • Alternative names
    • HCN 4 antibody
    • Hyperpolarization activated cyclic nucleotide gated potassium channel 4 antibody
    • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4 antibody

Images

  • ICC/IF image of ab66501 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66501, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • IHC image of ab66501 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66501, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Pai VP  et al. HCN4 ion channel function is required for early events that regulate anatomical left-right patterning in a nodal and lefty asymmetric gene expression-independent manner. Biol Open 6:1445-1457 (2017). Read more (PubMed: 28818840) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us. The molecular weight of IgG is 150 kDa with F(ab) Mwt 50 kda and 25 kDa weight of light chain. This is a complete antibody in whole antiserum, it is not a light chain antibody. I hope this information is helpful...

Read More

Thank you for contacting us. According to Swissprot the molecular weight of HCN4 protein is 129 kDa. Please be advised that the 129 kDa is the predicted molecular weight of the protein, the observed molecular weight will be either higher or less ba...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up