Overview

  • Product nameAnti-HCN4 antibody
    See all HCN4 primary antibodies
  • Description
    Rabbit polyclonal to HCN4
  • SpecificityReacts specifically with human 129 kDa protein
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the N terminal domain of human HCN4.

  • Positive control
    • ICC/IF: SKNSH cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab66501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/5000. Predicted molecular weight: 134 kDa.
ICC/IF 1/1000.

Target

  • RelevanceHCN4 is a member of the family of hyperpolarization activated and cyclic nucleotide gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.
  • Cellular localizationMembrane; multi pass membrane protein.
  • Database links
  • Alternative names
    • HCN 4 antibody
    • Hyperpolarization activated cyclic nucleotide gated potassium channel 4 antibody
    • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4 antibody

Anti-HCN4 antibody images

  • ICC/IF image of ab66501 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66501, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • IHC image of ab66501 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66501, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-HCN4 antibody (ab66501)

ab66501 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The molecular weight of IgG is 150 kDa with F(ab) Mwt 50 kda and 25 kDa weight of light chain. This is a complete antibody in whole antiserum, it is not a light chain antibody. I hope this information is helpful...

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Thank you for contacting us. According to Swissprot the molecular weight of HCN4 protein is 129 kDa. Please be advised that the 129 kDa is the predicted molecular weight of the protein, the observed molecular weight will be either higher or less ba...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"