Overview

  • Product nameAnti-HCN4 antibody [S114-10]
    See all HCN4 primary antibodies
  • Description
    Mouse monoclonal [S114-10] to HCN4
  • SpecificityNo cross reactivity against other HCNs.
  • Tested applicationsSuitable for: IHC-Fr, WB, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Rainbow Trout
  • Immunogen

    Fusion protein: PRGGLSPPGH SPGPPRTFPS APPRASGSHG SLLLPPASSP PPPQVPQRRG TPPLTPGRLT QDLKLISASQ PALPQDGAQT LRRASPHSSG, corresponding to cytoplasmic C terminal amino acids 1019-1108 of Rat HCN4 (Q9JKA7).

  • Positive control
    • COS cell lysate transiently transfected with HCN4.

Properties

Applications

Our Abpromise guarantee covers the use of ab85023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 129 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 0.1 - 1 µg/ml.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceHCN4 is a member of the family of hyperpolarization activated and cyclic nucleotide gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.
  • Cellular localizationMembrane; multi pass membrane protein.
  • Database links
  • Alternative names
    • HCN 4 antibody
    • Hyperpolarization activated cyclic nucleotide gated potassium channel 4 antibody
    • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4 antibody

Anti-HCN4 antibody [S114-10] images

  • ab85023 staining HCN4 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.

  • Overlay histogram showing SH-SY5Y cells stained with ab85023 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85023, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-HCN4 antibody [S114-10] (ab85023)

This product has been referenced in:
  • Mitrofanova LB  et al. Evidence of specialized tissue in human interatrial septum: histological, immunohistochemical and ultrastructural findings. PLoS One 9:e113343 (2014). IHC-P ; Human . Read more (PubMed: 25412099) »
  • Grunow B  et al. In vitro developed spontaneously contracting cardiomyocytes from rainbow trout as a model system for human heart research. Cell Physiol Biochem 27:1-12 (2011). ICC/IF ; Rainbow Trout . Read more (PubMed: 21325816) »

See all 3 Publications for this product

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Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (hESC-derived cardiomyocyte)
Specification hESC-derived cardiomyocyte
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Submitted Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"