Overview

  • Product nameAnti-HCST antibody
  • Description
    Goat polyclonal to HCST
  • SpecificityThis antibody is expected to recognise isoform 1 and may recognize isoform 2 lacking residue E81.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cynomolgus Monkey, Macaque Monkey
  • Immunogen

    Peptide with sequence

    C-RSPAQEDGKVYIN

    , from the C Terminus of the protein sequence according to NP_055081.1; NP_001007470.1.

  • Positive control
    • Human Spleen lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesPeptide ELISA: antibody detection limit dilution 1:128,000.
    WB: Use at a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 9 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHCST is a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. It may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D and may activate phosphatidylinositol 3-kinase dependent signaling pathways. The receptor complex containing HCST may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms.
    • Cellular localizationCell Membrane
    • Database links
    • Alternative names
      • DAP10 antibody
      • DNAX activation protein 10 antibody
      • Hematopoietic cell signal transducer antibody
      • KAP10 antibody
      • Membrane protein DAP10 antibody
      • phosphoinositide 3 kinase adaptor protein antibody
      • PIK3AP antibody
      • Transmembrane adapter protein KAP10 antibody
      see all

    Anti-HCST antibody images

    • Anti-HCST antibody (ab58596) at 0.2 µg/ml + Human spleen lysate at 35 µg

      Predicted band size : 9 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-HCST antibody (ab58596)

    ab58596 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab58596.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"