Overview

  • Product name
    Anti-HCST antibody
  • Description
    Goat polyclonal to HCST
  • Specificity
    This antibody is expected to recognise isoform 1 and may recognize isoform 2 lacking residue E81.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cynomolgus monkey, Macaque monkey
  • Immunogen

    Peptide with sequence

    C-RSPAQEDGKVYIN

    , from the C Terminus of the protein sequence according to NP_055081.1; NP_001007470.1.

  • Positive control
    • Human Spleen lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: antibody detection limit dilution 1:128,000.
    WB: Use at a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 9 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      HCST is a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. It may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D and may activate phosphatidylinositol 3-kinase dependent signaling pathways. The receptor complex containing HCST may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms.
    • Cellular localization
      Cell Membrane
    • Database links
    • Alternative names
      • DAP10 antibody
      • DNAX activation protein 10 antibody
      • Hematopoietic cell signal transducer antibody
      • KAP10 antibody
      • Membrane protein DAP10 antibody
      • phosphoinositide 3 kinase adaptor protein antibody
      • PIK3AP antibody
      • Transmembrane adapter protein KAP10 antibody
      see all

    Images

    • Anti-HCST antibody (ab58596) at 0.2 µg/ml + Human spleen lysate at 35 µg

      Predicted band size : 9 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    References

    ab58596 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab58596.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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