The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: antibody detection limit dilution 1:128,000.
WB: Use at a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 9 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHCST is a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. It may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D and may activate phosphatidylinositol 3-kinase dependent signaling pathways. The receptor complex containing HCST may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms.