Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)

Overview

  • Product nameAnti-HCV subtype 1b NS5B antibody [10D6]
    See all HCV subtype 1b NS5B primary antibodies
  • Description
    Mouse monoclonal [10D6] to HCV subtype 1b NS5B
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Hepatitis C Virus
  • Immunogen

    Recombinant fragment:

    LSSKAVNHIRSVWKDLLEDT

    , corresponding to amino acids 111-130 of HCV subtype 1b NS5B

  • Positive control
    • Huh7 cells harboring selectable subgenomic HCV RNA replicon

Properties

Applications

Our Abpromise guarantee covers the use of ab100895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 65 kDa.
IP Use at an assay dependent dilution.
ELISA 1/1000.

Target

  • Cellular localizationHost cytoplasm, nucleus, mitochondrion, endoplasmatic reticulum; secreted.
  • Database links
    • Alternative names
      • Hepacivirin antibody
      • NS3P antibody
      • POLG_HCVBK antibody
      • Serine protease NS3 antibody

    Anti-HCV subtype 1b NS5B antibody [10D6] images

    • IP was carried out with ab100895 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (-NS5B) and -Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with ab100895.
    • All lanes : Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)

      Lane 1 : Huh-7 transfected with HCV replicon
      Lane 2 : Huh-7 nontransfected cells


      Predicted band size : 65 kDa

    References for Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)

    This product has been referenced in:
    • Vogt DA  et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog 9:e1003302 (2013). WB . Read more (PubMed: 23593007) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (hepatoma cell line)
    Gel Running Conditions Reduced Denaturing (8%)
    Loading amount 30 µg
    Specification hepatoma cell line
    Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted May 16 2016

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (8%)
    Sample Hepatitis C Virus Cell lysate - whole cell (liver carcinoma cell)
    Specification liver carcinoma cell
    Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 29 2014

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab65410.

    To check the status of the order please contact o...

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    Thanks for your email.

    We do have a few additional HCV NS5B antibodies, but there is not one obvious, best choice. So I will mention 3 that you may find useful.
    ab35586 - most popular anti-HCV NS5B antibody, published in 5 papers. Tested...

    Read More

    Vielen Dank dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.



    Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten.

    Um Ihr Problem zu lösen, möchte ich die folgenden Vorschläge z...

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    DISCOUNT CODE: x
    Ablaufdatum: ddmm2012

    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab100895im Western blot oder der Immunfluoreszenz (IF) an HCV Genotyp 2a zu testen, annehmen möchten. Wie besprochen, ...

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    Vielen Dank dafür, dass Sie nachhaken.

    Leider haben wir noch keine guten Nachrichten für Sie:

    Wie versprochen haben wir uns in den entsprechenden Laboren erkundigt. Bislang haben wir für unsere anti-HCV NS5B-Antik&oum...

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    Thank you for contacting us. I am sorry for the delay in this response. ab100895 is a Mouse monoclonal antibody to Hepatitis C virus NS5B which has been used previously for ELISA and Western blotting. Unfortunately I have been unable to confirm if this...

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    Thank you for your patience on this matter. I have contacted our supplying laboratory on this matter and they have been unable to get back to me with any information regarding this question. I am sorry to say it does not appear that we have an answer f...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"