Anti-HCV subtype 1b NS5B antibody (ab35586)


  • Product nameAnti-HCV subtype 1b NS5B antibody
    See all HCV subtype 1b NS5B primary antibodies
  • Description
    Rabbit polyclonal to HCV subtype 1b NS5B
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Hepatitis C virus
  • Immunogen

    Recombinant fragment (Hepatitis C Virus) with a 21 amino acid C-terminal truncation



Our Abpromise guarantee covers the use of ab35586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/2000. Detects a band of approximately 64 kDa.


  • Cellular localizationHost cytoplasm, nucleus, mitochondrion, endoplasmatic reticulum; secreted.
  • Database links
    • Alternative names
      • Hepacivirin antibody
      • NS3P antibody
      • POLG_HCVBK antibody
      • Serine protease NS3 antibody

    Anti-HCV subtype 1b NS5B antibody images

    • All lanes : Anti-HCV subtype 1b NS5B antibody (ab35586) at 1/2000 dilution

      Lane 1 : Lysate from Huh7 cells, mock transfection
      Lane 2 : Lysate from Huh7 cells transfected with NS5B

      Observed band size : 64 kDa (why is the actual band size different from the predicted?)

    References for Anti-HCV subtype 1b NS5B antibody (ab35586)

    This product has been referenced in:
    • Carocci M  et al. The bioactive lipid 4-hydroxyphenyl retinamide inhibits flavivirus replication. Antimicrob Agents Chemother 59:85-95 (2015). Read more (PubMed: 25313218) »
    • Shwetha S  et al. HuR Displaces Polypyrimidine Tract Binding Protein To Facilitate La Binding to the 3' Untranslated Region and Enhances Hepatitis C Virus Replication. J Virol 89:11356-71 (2015). WB ; Human . Read more (PubMed: 26339049) »

    See all 11 Publications for this product

    Product Wall

    Thank you for contacting us.

    The lab sent me the correct immunogen info:
    "Recombinant HCV (Hepatitis C Virus) NS5B with a 21 amino acid C-terminal truncation, genotype 1b, clone Con1, UniProt link

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    Vielen Dank dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

    Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten.

    Um Ihr Problem zu lösen, möchte ich die folgenden Vorschläge z...

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    Vielen Dank dafür, dass Sie nachhaken.

    Leider haben wir noch keine guten Nachrichten für Sie:

    Wie versprochen haben wir uns in den entsprechenden Laboren erkundigt. Bislang haben wir für unsere anti-HCV NS5B-Antik&oum...

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    Thank you for contacting us. I am sorry for the delay in this response. ab100895 is a Mouse monoclonal antibody to Hepatitis C virus NS5B which has been used previously for ELISA and Western blotting. Unfortunately I have been unable to confirm if this...

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    Application ELISA
    Sample Human Cell (huh-7 hepatocarcinoma cells)
    Specification huh-7 hepatocarcinoma cells
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
    Type Sandwich (Capture)

    Abcam user community

    Verified customer

    Submitted Jun 17 2008

    Thank you for your enquiry. I can confirm that this antibody works well with both 1a and 1b genotypes and also should work with many other genotypes as it is a polyclonal raised to an almost full-length protein. I hope this information is helpf...

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    Thank you for your patience. Regarding ab35586 (Hepatitis C virus NS5B antibody), this antibody will recognize genotype 1a. Please don't hesitate to contact us again if you have further questions.