Anti-HCV subtype 1b NS5B antibody (ab35586)

Overview

  • Product name
    Anti-HCV subtype 1b NS5B antibody
    See all HCV subtype 1b NS5B primary antibodies
  • Description
    Rabbit polyclonal to HCV subtype 1b NS5B
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Hepatitis C virus
  • Immunogen

    Recombinant fragment (Hepatitis C Virus) with a 21 amino acid C-terminal truncation

Properties

Applications

Our Abpromise guarantee covers the use of ab35586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/2000. Detects a band of approximately 64 kDa.

Target

  • Cellular localization
    Host cytoplasm, nucleus, mitochondrion, endoplasmatic reticulum; secreted.
  • Database links
    • Alternative names
      • Hepacivirin antibody
      • NS3P antibody
      • POLG_HCVBK antibody
      • Serine protease NS3 antibody

    Images

    • All lanes : Anti-HCV subtype 1b NS5B antibody (ab35586) at 1/2000 dilution

      Lane 1 : Lysate from Huh7 cells, mock transfection
      Lane 2 : Lysate from Huh7 cells transfected with NS5B


      Observed band size : 64 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Vegna S  et al. NOD1 Participates in the Innate Immune Response Triggered by Hepatitis C Virus Polymerase. J Virol 90:6022-35 (2016). Read more (PubMed: 27099311) »
    • Valero ML  et al. Hepatitis C Virus RNA-Dependent RNA Polymerase Interacts with the Akt/PKB Kinase and Induces Its Subcellular Relocalization. Antimicrob Agents Chemother 60:3540-50 (2016). Read more (PubMed: 27021315) »

    See all 13 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting us.

    The lab sent me the correct immunogen info:
    "Recombinant HCV (Hepatitis C Virus) NS5B with a 21 amino acid C-terminal truncation, genotype 1b, clone Con1, UniProt link
    http://www.uniprot.org/uniprot/?query...

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    Vielen Dank dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.



    Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten.

    Um Ihr Problem zu lösen, möchte ich die folgenden Vorschläge z...

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    Vielen Dank dafür, dass Sie nachhaken.

    Leider haben wir noch keine guten Nachrichten für Sie:

    Wie versprochen haben wir uns in den entsprechenden Laboren erkundigt. Bislang haben wir für unsere anti-HCV NS5B-Antik&oum...

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    Thank you for contacting us. I am sorry for the delay in this response. ab100895 is a Mouse monoclonal antibody to Hepatitis C virus NS5B which has been used previously for ELISA and Western blotting. Unfortunately I have been unable to confirm if this...

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    Application
    ELISA
    Sample
    Human Cell (huh-7 hepatocarcinoma cells)
    Specification
    huh-7 hepatocarcinoma cells
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
    Type
    Sandwich (Capture)
    Username

    Abcam user community

    Verified customer

    Submitted Jun 17 2008

    Thank you for your enquiry. I can confirm that this antibody works well with both 1a and 1b genotypes and also should work with many other genotypes as it is a polyclonal raised to an almost full-length protein. I hope this information is helpf...

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    Thank you for your patience. Regarding ab35586 (Hepatitis C virus NS5B antibody), this antibody will recognize genotype 1a. Please don't hesitate to contact us again if you have further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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