Overview

  • Product nameAnti-HDAC1 antibody [1.T.9] - ChIP GradeSee all HDAC1 primary antibodies ...
  • Description
    Mouse monoclonal [1.T.9] to HDAC1 - ChIP Grade
  • Tested applicationsWB, ICC, IP, ChIP, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
    Predicted to work with: Rat, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    CEEKPEAKGVKEEVKLA

    conjugated to KLH, corresponding to amino acids 467-482 of Mouse HDAC1

  • Positive control
    • 3T3/A31 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab31263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 60 kDa.
ICC Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration. PubMed: 22416134
IHC-FoFr 1/1000. PubMed: 19424149

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686H12203 antibody
    • GON 10 antibody
    • HD 1 antibody
    • HD1 antibody
    • HD1 antibody
    • HDAC 1 antibody
    • HDAC1 antibody
    • hdac1: histone deacetylase 1 antibody
    • hdac1: histone deacetylase 1 antibody
    • HDAC1_HUMAN antibody
    • Histone deacetylase 1 (HD1) antibody
    • Histone deacetylase 1 (HD1) antibody
    • Histone deacetylase 1 antibody
    • Reduced potassium dependency yeast homolog like 1 antibody
    • RPD3 (reduced potassium dependency yeast homolog) like 1 antibody
    • RPD3 (reduced potassium dependency) antibody
    • RPD3 (reduced potassium dependency) antibody
    • RPD3 antibody
    • RPD3 antibody
    • RPD3L1 antibody
    • RPD3L1 antibody
    see all

Anti-HDAC1 antibody [1.T.9] - ChIP Grade images

  • Anti-HDAC1 antibody [1.T.9] - ChIP Grade (ab31263) at 0.1 µg/ml + 3T3 cell lysate

    Predicted band size : 60 kDa

References for Anti-HDAC1 antibody [1.T.9] - ChIP Grade (ab31263)

This product has been referenced in:
  • Martínez FP & Tang Q Leucine zipper domain is required for Kaposi sarcoma-associated herpesvirus (KSHV) K-bZIP protein to interact with histone deacetylase and is important for KSHV replication. J Biol Chem 287:15622-34 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22416134) »
  • Kurita M  et al. HDAC2 regulates atypical antipsychotic responses through the modulation of mGlu2 promoter activity. Nat Neurosci 15:1245-54 (2012). Read more (PubMed: 22864611) »

See all 8 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"