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CEEKPEAKGVKEEVKLAconjugated to KLH, corresponding to amino acids 467-482 of Mouse HDAC1
Our Abpromise guarantee covers the use of ab31263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 60 kDa.|
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22416134|
|IHC-FoFr||1/1000. PubMed: 19424149|
ab31263 staining HDAC1 in COS7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in 2% BSA) for 1 hour at 25°C. A DyLight® 488-conjugated goat anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.
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