Anti-HDAC1 antibody - ChIP Grade (ab1767)
Key features and details
- Rabbit polyclonal to HDAC1 - ChIP Grade
- Suitable for: WB, ICC/IF, ChIP, IP, IHC - Wholemount
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-HDAC1 antibody - ChIP Grade
See all HDAC1 primary antibodies -
Description
Rabbit polyclonal to HDAC1 - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, ChIP, IP, IHC - Wholemountmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
Synthetic peptide:
C-SGSGTGSGAGAKGAKENNI
, corresponding to amino acids 503-521 of Fruit fly (Drosophila melanogaster) HDAC1 -
General notes
The SIN3/RPD3 deacetylase complex is essential for G(2) phase cell cycle progression and regulation of SMRTER corepressor levels.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading...
-
Purity
Whole antiserum -
Primary antibody notes
The SIN3/RPD3 deacetylase complex is essential for G(2) phase cell cycle progression and regulation of SMRTER corepressor levels. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Assay kits
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
|
ChIP |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
|
IHC - Wholemount |
Use at an assay dependent concentration. PubMed: 22195015
|
Notes |
---|
WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC - Wholemount
Use at an assay dependent concentration. PubMed: 22195015 |
Target
-
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. -
Tissue specificity
Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 38565 Drosophila melanogaster
-
Alternative names
- DKFZp686H12203 antibody
- GON 10 antibody
- HD1 antibody
see all
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (15)
ab1767 has been referenced in 15 publications.
- Song Z et al. eIF3j inhibits translation of a subset of circular RNAs in eukaryotic cells. Nucleic Acids Res 50:11529-11549 (2022). PubMed: 36330957
- Beaver M et al. Chromatin and transcriptomic profiling uncover dysregulation of the Tip60 HAT/HDAC2 epigenomic landscape in the neurodegenerative brain. Epigenetics N/A:1-22 (2021). PubMed: 34369292
- Beaver M et al. Disruption of Tip60 HAT mediated neural histone acetylation homeostasis is an early common event in neurodegenerative diseases. Sci Rep 10:18265 (2020). PubMed: 33106538
- Panikker P et al. Restoring Tip60 HAT/HDAC2 Balance in the Neurodegenerative Brain Relieves Epigenetic Transcriptional Repression and Reinstates Cognition. J Neurosci 38:4569-4583 (2018). PubMed: 29654189
- Cabezas-Cruz A et al. Anaplasma phagocytophilum increases the levels of histone modifying enzymes to inhibit cell apoptosis and facilitate pathogen infection in the tick vector Ixodes scapularis. Epigenetics 11:303-19 (2016). PubMed: 27019326