Overview

  • Product nameAnti-HDAC1 antibody - ChIP GradeSee all HDAC1 primary antibodies ...
  • Description
    Rabbit polyclonal to HDAC1 - ChIP Grade
  • Tested applicationsICC/IF, ChIP, IP, WBmore details
  • Species reactivity
    Reacts with: Fruit fly (Drosophila melanogaster)
  • Immunogen

    Synthetic peptide:

    C-SGSGTGSGAGAKGAKENNI

    , corresponding to amino acids 503-521 of Fruit fly (Drosophila melanogaster) HDAC1

  • General notes


    The SIN3/RPD3 deacetylase complex is essential for G(2) phase cell cycle progression and regulation of SMRTER corepressor levels.

Properties

Applications

Our Abpromise guarantee covers the use of ab1767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent dilution.
WB 1/400. Predicted molecular weight: 65 kDa.

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • DKFZp686H12203 antibody
      • GON 10 antibody
      • HD 1 antibody
      • HD1 antibody
      • HDAC 1 antibody
      • HDAC1 antibody
      • hdac1: histone deacetylase 1 antibody
      • HDAC1_HUMAN antibody
      • Histone deacetylase 1 (HD1) antibody
      • Histone deacetylase 1 antibody
      • Reduced potassium dependency yeast homolog like 1 antibody
      • reduced potassium dependency yeast homolog-like 1 antibody
      • RPD3 (reduced potassium dependency yeast homolog) like 1 antibody
      • RPD3 (reduced potassium dependency) antibody
      • RPD3 antibody
      • RPD3L1 antibody
      see all

    Anti-HDAC1 antibody - ChIP Grade images

    • Immunohistochemical analysis of whole mount Drosophila melanogaster brain, staining HDAC1 with ab1767, at 1/1000 dilution.

    References for Anti-HDAC1 antibody - ChIP Grade (ab1767)

    This product has been referenced in:
    • Ji Z  et al. The forkhead transcription factor FOXK2 acts as a chromatin targeting factor for the BAP1-containing histone deubiquitinase complex. Nucleic Acids Res 42:6232-42 (2014). WB . Read more (PubMed: 24748658) »
    • Fitzsimons HL  et al. The histone deacetylase HDAC4 regulates long-term memory in Drosophila. PLoS One 8:e83903 (2013). ICC/IF ; Fruit fly (Drosophila melanogaster) . Read more (PubMed: 24349558) »

    See all 7 Publications for this product

    Product Wall

    Thank you for your enquiry. This antibody is sold as whole antiserum. The IgG concentration has not been determined. However, I would expect it to be within a range of 10-16mgml-1 given that it is rabbit whole serum. We have not received reviews of ...

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    Thank you for your enquiry. All the information that we have regarding ab1767 is located on the online datasheet. Unfortunately, we are not aware of any publications that feature the use of this antibody. To our knowledge, customized protocols are no...

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    The expected band size is 65 kDa. I would suggest that perhaps you should try using a dilution at 1:100.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"