Overview

  • Product name
    Anti-HDAC1 antibody - ChIP Grade
    See all HDAC1 primary antibodies
  • Description
    Rabbit polyclonal to HDAC1 - ChIP Grade
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ChIP/Chip, ChIP, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human HDAC1 aa 466-482 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    C-KEEKPEAKGVKEEVKLA


    Database link: HDAC1

  • Positive control
    • HeLa human epithelioid carcinoma cells. Whole cell lysate from human HEK293 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab7028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/20000. Predicted molecular weight: 55 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
ChIP/Chip Use at an assay dependent concentration.
ChIP Use 5µl for 106 cells.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 23911933

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • Tissue specificity
    Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686H12203 antibody
    • GON 10 antibody
    • HD1 antibody
    • HDAC 1 antibody
    • HDAC1 antibody
    • HDAC1_HUMAN antibody
    • Histone deacetylase 1 antibody
    • Reduced potassium dependency yeast homolog like 1 antibody
    • RPD3 antibody
    • RPD3L1 antibody
    see all

Images

  •  Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab7028 to HDAC1 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5 µl of ab7028 and 2x10.

  • All lanes : Anti-HDAC1 antibody - ChIP Grade (ab7028) at 1/1000 dilution

    Lane 1 : Whole cell lysate from human HEK293 cell line
    Lane 2 : Whole cell lysate from human HEK293 cell line treated with HDAC1 gene silencing shRNA

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit Ig at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa


    Exposure time: 10 seconds

    See Abreview

  • Immunofluorescence analysis of methanol fixed HeLa cells with ab7028 labelling HDAC1 at 1:200 dilution. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

  • Immunohistochemical analysis of paraformaldehyde fixed paraffin embedded human breast tumour section with ab7028 labelling HDAC1 at 1/500 dilution.

    See Abreview

  • Anti-HDAC1 antibody - ChIP Grade (ab7028) at 1/2000 dilution + Human HuH-7 whole cell lysate at 15 µg

    Secondary
    HRP conjugated Sheet anti-rabbit IgG polyclonal at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa


    Exposure time: 20 seconds


    Blocked with 5% milk for 1 hour at 20°C

    See Abreview

  • HDAC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7028.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 60ka: HDAC1.

References

This product has been referenced in:
  • Tosic M  et al. Lsd1 regulates skeletal muscle regeneration and directs the fate of satellite cells. Nat Commun 9:366 (2018). Read more (PubMed: 29371665) »
  • Zhao M  et al. IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation. Nat Commun 9:583 (2018). ChIP . Read more (PubMed: 29422534) »

See all 128 Publications for this product

Customer reviews and Q&As

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Application
ChIP
Sample
Mouse Tissue lysate - other (colonic crypts)
Specification
colonic crypts
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldhyde
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Submitted Oct 06 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Sep 28 2015

Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Human Cell lysate - whole cell (Huh7 liver)
Specification
Huh7 liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jan 26 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Forebrain Tissue)
Loading amount
200 µg
Specification
Forebrain Tissue
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Nov 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7 cell line)
Loading amount
15 µg
Specification
huh-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted May 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Glioma cell line C6)
Specification
Glioma cell line C6
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton X 100
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
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Submitted Aug 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line)
Specification
human glioblastoma cell line
Fixative
Formaldehyde
Permeabilization
Yes - 0,1% Triton X-100
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
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Submitted Jun 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line)
Specification
human glioblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - Tritonx100 0.1% in PBS
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
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Submitted Apr 26 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - whole cell (lung carcinoma)
Specification
lung carcinoma
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Real-time PCR
Negative control
rabbit IgG
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Submitted Apr 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoblastoid cell line)
Loading amount
20 µg
Specification
lymphoblastoid cell line
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 02 2010

1-10 of 23 Abreviews

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