Overview

  • Product nameAnti-HDAC1 antibody [EPR460(2)]
    See all HDAC1 primary antibodies
  • Description
    Rabbit monoclonal [EPR460(2)] to HDAC1
  • Tested applicationsICC, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human HDAC1.

  • Positive control
    • Jurkat, HeLa, K562, and MCF7 cell lysates; Human colon tissue; Human tonsil tissue
  • General notes

    Produced under U.S. Patent No. 5,675,063.

    This product is available conjugated to DyLight® 488 see ab139939.

    A 40 μl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/250 - 1/500.
ICC/IF 1/400.

Use with paraformaldehyde fixed cells.

WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.Can be blocked with HDAC1 peptide (ab174565).
IHC-P 1/50 - 1/100.

Antigen retrieval is recommended.

  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
    • Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
    • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
    • Post-translational
      modifications
      Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
      Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
      Ubiquitinated by CHFR, leading to its degradation by the proteasome.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686H12203 antibody
      • GON 10 antibody
      • HD1 antibody
      • HDAC 1 antibody
      • HDAC1 antibody
      • HDAC1_HUMAN antibody
      • Histone deacetylase 1 antibody
      • Reduced potassium dependency yeast homolog like 1 antibody
      • RPD3 antibody
      • RPD3L1 antibody
      see all

    Anti-HDAC1 antibody [EPR460(2)] images

    • ab109411 (1/400) staining HDAC1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview

    • All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 55 kDa
    • Immunohistochemical analysis of HDAC1 in paraffin-embedded Human tonsil tissue using ab109411 at 1/50 dilution.
    • Immunohistochemical analysis of HDAC1 in paraffin-embedded Human colon tissue using ab109411 at 1/50 dilution.

    References for Anti-HDAC1 antibody [EPR460(2)] (ab109411)

    This product has been referenced in:
    • Zhang W  et al. ELL inhibits E2F1 transcriptional activity by enhancing E2F1 deacetylation via recruitment of histone deacetylase 1. Mol Cell Biol 34:765-75 (2014). Read more (PubMed: 24344198) »
    • Klinz FJ  et al. Histone deacetylases 2 and 9 are coexpressed and nuclear localized in human molar odontoblasts in vivo. Histochem Cell Biol : (2012). IHC-FrFl ; Human . Read more (PubMed: 22297573) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton-X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted May 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"