Anti-HDAC1 antibody [EPR460(2)] (ab109411)
- Product nameAnti-HDAC1 antibody [EPR460(2)]See all HDAC1 primary antibodies ...
- DescriptionRabbit monoclonal [EPR460(2)] to HDAC1
- Tested applicationsICC/IF, WB, IHC-P, ICC more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues near the C-terminus of Human HDAC1.
- Positive control
- Jurkat, HeLa, K562, and MCF7 cell lysates; Human colon tissue; Human tonsil tissue
- General notes
Produced under U.S. Patent No. 5,675,063.
This product is available conjugated to DyLight® 488 see ab139939.
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR460(2)
- Research Areas
Our Abpromise guarantee covers the use of ab109411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: 1/400. Use with paraformaldehyde fixed cells.|
|WB||WB: 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.Can be blocked with HDAC1 peptide (ab174565).|
|IHC-P||IHC-P: 1/50 - 1/100. Antigen retrieval is recommended|
|ICC||ICC: 1/250 - 1/500.|
- FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
- Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
- Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome.
- Cellular localizationNucleus.
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Anti-HDAC1 antibody [EPR460(2)] images
All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 55 kDa
Immunohistochemical analysis of HDAC1 in paraffin-embedded Human colon tissue using ab109411 at 1/50 dilution.
ab109411 (1/400) staining HDAC1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
Immunohistochemical analysis of HDAC1 in paraffin-embedded Human tonsil tissue using ab109411 at 1/50 dilution.
References for Anti-HDAC1 antibody [EPR460(2)] (ab109411)
This product has been referenced in:
- Klinz FJ et al. Histone deacetylases 2 and 9 are coexpressed and nuclear localized in human molar odontoblasts in vivo. Histochem Cell Biol : (2012). IHC-FrFl ; Human . Read more (PubMed: 22297573) »
- Kaller M et al. Genome-wide characterization of miR-34a induced changes in protein and mRNA expression by a combined pulsed SILAC and microarray analysis. Mol Cell Proteomics 10:M111.010462 (2011). WB ; Human . Read more (PubMed: 21566225) »