Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab41407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.05 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Can be blocked with Zebrafish HDAC1 peptide (ab41406).
IP Use at an assay dependent concentration.

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686H12203 antibody
    • GON 10 antibody
    • HD 1 antibody
    • HD1 antibody
    • HD1 antibody
    • HDAC 1 antibody
    • HDAC1 antibody
    • hdac1: histone deacetylase 1 antibody
    • hdac1: histone deacetylase 1 antibody
    • HDAC1_HUMAN antibody
    • Histone deacetylase 1 (HD1) antibody
    • Histone deacetylase 1 (HD1) antibody
    • Histone deacetylase 1 antibody
    • Reduced potassium dependency yeast homolog like 1 antibody
    • RPD3 (reduced potassium dependency yeast homolog) like 1 antibody
    • RPD3 (reduced potassium dependency) antibody
    • RPD3 (reduced potassium dependency) antibody
    • RPD3 antibody
    • RPD3 antibody
    • RPD3L1 antibody
    • RPD3L1 antibody
    see all

Anti-HDAC1 antibody - Loading Control images

  • Anti-HDAC1 antibody - Loading Control (ab41407) at 1 µg/ml + Zebrafish Embryo Extract at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa


    Exposure time : 2 minutes
  • Anti-HDAC1 antibody - Loading Control (ab41407) at 0.05 µg/ml + whole embryo zebrafish tissue lysate at 45 µg

    Secondary
    Donkey Anti Rabbit IgG (H+L) Conjugation: HRP at 1/5000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 35 kDa (possible cross reactivity).

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-HDAC1 antibody - Loading Control (ab41407)

This product has been referenced in:
  • Tittle RK  et al. Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens. Dev Biol 350:50-63 (2011). WB ; Zebrafish . Read more (PubMed: 21126517) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Zebrafish Tissue lysate - whole (whole embryos)
Total protein in input 1000 µg
Specification whole embryos
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Apr 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (whole embryo)
Loading amount 45 µg
Specification whole embryo
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"