Anti-HDAC1 antibody [mAbcam 68436] - Nuclear Loading Control (ab68436)
Key features and details
- Mouse monoclonal [mAbcam 68436] to HDAC1 - Nuclear Loading Control
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-HDAC1 antibody [mAbcam 68436] - Nuclear Loading Control
See all HDAC1 primary antibodies -
Description
Mouse monoclonal [mAbcam 68436] to HDAC1 - Nuclear Loading Control -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Cow, Human, Orangutan -
Immunogen
Synthetic peptide corresponding to Human HDAC1 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab20434) -
Positive control
- WB: IOUD2 Whole Cell Lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
mAbcam 68436 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
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Notes |
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa). |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. -
Tissue specificity
Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 404126 Cow
- Entrez Gene: 3065 Human
- Entrez Gene: 433759 Mouse
- Entrez Gene: 100172663 Orangutan
- Entrez Gene: 297893 Rat
- Omim: 601241 Human
- SwissProt: Q32PJ8 Cow
- SwissProt: Q13547 Human
see all -
Alternative names
- DKFZp686H12203 antibody
- GON 10 antibody
- HD1 antibody
see all
Images
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Anti-HDAC1 antibody [mAbcam 68436] - Nuclear Loading Control (ab68436) at 5 µg/ml + IOUD2 Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Datasheets and documents
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Datasheet download
References (2)
ab68436 has been referenced in 2 publications.
- Asadian P et al. The expression profile of sterile alpha motif and histidine-aspartate domain-containing protein 1 (SAMHD1) in feline tissues. Vet Immunol Immunopathol 195:7-18 (2018). WB . PubMed: 29249320
- Remes Lenicov F et al. Prostaglandin E2 Antagonizes TGF-ß Actions During the Differentiation of Monocytes Into Dendritic Cells. Front Immunol 9:1441 (2018). PubMed: 29988364