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Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners.
HDAC1 is a class I histone deacetylase that plays a critical role in transcriptional repression of gene expression and has been shown to be an integral component of multiprotein co-repressor complexes. It has been observed that an important mechanism of HDAC1 activity regulation is sumoylation, which acts by potentiating HDAC1 activity.
Abcam's HDAC1 Sumoylation Assay Kit (ab115132) allows the user to measure in vivo HDAC1 sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo HDAC1 sumoylation from multiple mammalian cells/tissues extracts.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 25ml||1 x 50ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|8-Well Control Strips||2 units||3 units|
|Color Development Solution||1 x 6ml||1 x 12ml|
|Signal Enhancer||1 x 120µl||1 x 240µl|
|Signal Report Solution||1 x 10µl||1 x 20µl|
|Stop Solution||1 x 3ml||1 x 6ml|
|Sumo Antibody, 1µg/µL||1 x 5µl||1 x 10µl|
|Sumo Assay Buffer||1 x 2ml||1 x 4ml|
|Sumo Protein, 1 µg/µL||1 x 6µl||1 x 12µl|
Our Abpromise guarantee covers the use of ab115132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115132 has not yet been referenced specifically in any publications.