Recombinant
RabMAb

Anti-HDAC11 antibody [EPR11342(B)] (ab166907)

Overview

  • Product name
    Anti-HDAC11 antibody [EPR11342(B)]
    See all HDAC11 primary antibodies
  • Description
    Rabbit monoclonal [EPR11342(B)] to HDAC11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human HDAC11 (UniProt Q96DB2).

  • Positive control
    • Jurkat, HCT-116, MCF7, HepG2 , PC-12 (Rat adrenal gland pheochromocytoma), C6 (Rat glial tumor glial cell) cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    • Tissue specificity
      Weakly expressed in most tissues. Strongly expressed in brain, heart, skeletal muscle, kidney and testis.
    • Sequence similarities
      Belongs to the histone deacetylase family.
    • Cellular localization
      Nucleus. Predominantly nuclear.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ22237 antibody
      • HD 11 antibody
      • HD11 antibody
      • HDA11_HUMAN antibody
      • HDAC 11 antibody
      • HDAC11 antibody
      • Histone deacetylase 11 antibody
      see all

    Images

    • ab166907 (purified) at 1:30 dilution (2ug) immunoprecipitating HDAC11 in HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate.
      Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate,10ug
      Lane 2 (+): ab166907 & HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab166907 in HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate.
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-HDAC11 antibody [EPR11342(B)] (ab166907) at 1/10000 dilution (purified)

      Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
      Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 39 kDa
      Observed band size: 40 kDa (why is the actual band size different from the predicted?)



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-HDAC11 antibody [EPR11342(B)] (ab166907) at 1/1000 dilution (purified)

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
      Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 39 kDa
      Observed band size: 40 kDa (why is the actual band size different from the predicted?)



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-HDAC11 antibody [EPR11342(B)] (ab166907) at 1/1000 dilution (unpurified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : HCT-116 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 39 kDa

    • Detection of HDAC11 by western blot analysis of immunoprecipitation pellet from Jurkat cell lysate using unpurified ab166907 at 1/10.
      Secondary antibody was HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:
    • Mondello P  et al. Panobinostat acts synergistically with ibrutinib in diffuse large B cell lymphoma cells with MyD88 L265 mutations. JCI Insight 2:e90196 (2017). WB ; Human . Read more (PubMed: 28352655) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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